LOCUS       sequence-126219-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration of 2GFP tag at Atg20 C terminus, use auxotrophic marker 
            URA3(Candida albicans).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: LD220
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(61..777)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(64..777)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ctaatttaat aataaaaatc ataaatcata agaaattcgc ttatttagaa gtggcgcgcc
       61 ttatttatat aattcatcca taccatgggt aataccagca gcagtaacaa attctaacaa
      121 gaccatgtgg tctctctttt cgtttggatc tttggataag gcagattgag tggataagta
      181 atggttgtct ggtaacaaga ctggaccatc accaattgga gtattttgtt gataatggtc
      241 agctaattga acagaaccat cttcaatgtt gtgtctaatt ttgaagttaa ctttgatacc
      301 attcttttgt ttgtcagcca tgatgtaaac attgtgagag ttatagttgt attccaattt
      361 gtgacctaaa atgttaccat cttctttaaa atcaatacct tttaattcga ttctattaac
      421 taaggtatca ccttcaaact tgacttcagc tctggtcttg tagttaccgt catctttgaa
      481 aaaaatagtt ctttcttgaa cataaccttc tggcatggca gacttgaaaa agtcatgttg
      541 tttcatatga tctgggtatc tagaaaaaca ttgaacacca taagttaaag tagtgactaa
      601 ggttggccat ggaactggca atttaccagt agtacaaata aattttaagg tcaatttacc
      661 gtaagtagca tcaccttcac cttcaccgga gacagaaaat ttgtgaccat taacatcacc
      721 atctaattca accaaaattg ggacaacacc agtgaataat tcttcacctt tagacatgaa
      781 tggcttctct agatcacgcg ttggaactgg aattttgtac aattcatcaa taccatgggt
      841 aataccagca gcagta
//
