LOCUS       sequence-12622-e        5845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Mitchell T, Sugden B
  TITLE     Stimulation of NF-kappa B-mediated transcription by mutant 
            derivatives of the latent membrane protein of Epstein-Barr virus.
  JOURNAL   J Virol. 1995 May . 69(5):2968-76.
  PUBMED    7707523
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1995 May . 69(5):2968-76."
COMMENT     Sequence Label: p1242 3x-KB-L
FEATURES             Location/Qualifiers
     source          1..5845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(257..268)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             324..1976
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(390..408)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2216..2281
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2411..2431
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2856..2990
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3132..3149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3303..3891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3383..3402)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4062..4853,4854..4922))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4685..4704
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4923..5027)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5054..5509
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5141..5160)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5351..5372
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5699..5820
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccagcttgg gctgcaggtc gaggctgggg attcccatcc tcgaggctgg ggattcccca
       61 tctcgaggct ggggattccc catctcgagg ctggggattc cccatctcga ccgtccatcc
      121 attcacagcg cttctataaa ggcgccagct gaggcgccta ctactccaac cgcgactgca
      181 gcgagcaact gagaagactg gatagagccg gcggttccgc gaacgagcag tgaccgcgct
      241 cccacccagc tctgctctgc agctcccacc agtgtctctc tagaggatct aagtaagctt
      301 ggcattccgg tactgttggt aaaatggaag acgccaaaaa cataaagaaa ggcccggcgc
      361 cattctatcc tctagaggat ggaaccgctg gagagcaact gcataaggct atgaagagat
      421 acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg aacatcacgt
      481 acgcggaata cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga
      541 atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt
      601 tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg
      661 aattgctcaa cagtatgaac atttcgcagc ctaccgtagt gtttgtttcc aaaaaggggt
      721 tgcaaaaaat tttgaacgtg caaaaaaaat taccaataat ccagaaaatt attatcatgg
      781 attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac
      841 ctcccggttt taatgaatac gattttgtac cagagtcctt tgatcgtgac aaaacaattg
      901 cactgataat gaattcctct ggatctactg ggttacctaa gggtgtggcc cttccgcata
      961 gaactgcctg cgtcagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc
     1021 cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac
     1081 tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt
     1141 ttttacgatc ccttcaggat tacaaaattc aaagtgcgtt gctagtacca accctatttt
     1201 cattcttcgc caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg
     1261 cttctggggg cgcacctctt tcgaaagaag tcggggaagc ggttgcaaaa cgcttccatc
     1321 ttccagggat acgacaagga tatgggctca ctgagactac atcagctatt ctgattacac
     1381 ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg
     1441 ttgtggatct ggataccggg aaaacgctgg gcgttaatca gagaggcgaa ttatgtgtca
     1501 gaggacctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg
     1561 acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct
     1621 tcatagttga ccgcttgaag tctttaatta aatacaaagg atatcaggtg gcccccgctg
     1681 aattggaatc gatattgtta caacacccca acatcttcga cgcgggcgtg gcaggtcttc
     1741 ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga
     1801 tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc
     1861 gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa
     1921 gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagtccaaa ttgtaaaatg
     1981 taactgtatt cagcgatgac gaaattctta gctattgtaa tactgcgatg agtggcaggg
     2041 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga
     2101 ataagtgata ataagcggat gaatggcaga aattcgccgg atctttgtga aggaacctta
     2161 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     2221 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     2281 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
     2341 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
     2401 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
     2461 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
     2521 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
     2581 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
     2641 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
     2701 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
     2761 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2821 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     2881 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2941 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgtcgaccga
     3001 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     3061 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     3121 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     3181 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3241 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3301 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3361 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3421 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3481 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3541 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3601 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3661 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3721 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     3781 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3841 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3901 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3961 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4021 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4081 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4141 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4201 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4261 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4321 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4381 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4441 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4501 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4561 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4621 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4681 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4741 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4801 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4861 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4921 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4981 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5041 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5101 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5161 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5221 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5281 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5341 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5401 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5461 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ccattcgcca
     5521 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     5581 cccaagctac catgataagt aagtaatatt aaggtacgtg gaggttttac ttgctttaaa
     5641 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     5701 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5761 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5821 tggtactgta actgagctaa cataa
//
