LOCUS       sequence-126222-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 4YZ8
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(774..794)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(807..824)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             834..856
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    871..895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(895..914)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(896..914)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttc gtcatcatcc caggaagccc gaacagttat cagcaccgca gtgacattcg
      121 gtacggccat tccccaggca atccaggtta taattaaagg tcagctccat accagcagga
      181 atgtcgcaca gggcaaacag gccgacgcgc acatccccgt taaccgtcca tttttgggtt
      241 tcgcaattcg ggttacagga gtgattcata aaacgtgaat agttaccctt cgggcccgcg
      301 tcgataatgc gatcttttgt tacagtcagc atgtaaaaat tcgtcacgct gttctcgtga
      361 gcacgtttga tacgcagacg gcactcttct tcgtcaatca gctcaccgac atattcatta
      421 acgaactcgc cctttttgat agagcgtttg gtacgcagcc cccagccgcg acgttctgtc
      481 ttaataattt ctgcatccgg gtacagacgt ttggtaaaac actggttctg gcaacgatcg
      541 ccggccggac acacttgcgg gtggcactca tactgcagca tacgattcag acattcgctc
      601 tccagaccgc aaggattttc gtccgccggt ttacaattgc aacgcggaat ttccgacaga
      661 tcagctactt ggatctggac cttgccaata actttgtttg ctttgatgtg cttgtacggc
      721 ggcggtttgc gagagttctt ttcgatttcc agtgcttcct tactctcacg ctggccctgg
      781 aaatacaagt tttctctgcc gctgctgtga tgatgatgat gatgcatggt atatctcctt
      841 cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat
      901 agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcggt
      961 gggcccggcc attcaaccgg gg
//
