LOCUS       sequence-126223-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 4YZ8
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             827..844
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(897..915)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      911..958
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg catcatcatc
       61 atcatcacag cagcggcaga gaaaacttgt atttccaggg ccagcgtgag agtaaggaag
      121 cactggaaat cgaaaagaac tctcgcaaac cgccgccgta caagcacatc aaagcaaaca
      181 aagttattgg caaggtccag atccaagtag ctgatctgtc ggaaattccg cgttgcaatt
      241 gtaaaccggc ggacgaaaat ccttgcggtc tggagagcga atgtctgaat cgtatgctgc
      301 agtatgagtg ccacccgcaa gtgtgtccgg ccggcgatcg ttgccagaac cagtgtttta
      361 ccaaacgtct gtacccggat gcagaaatta ttaagacaga acgtcgcggc tgggggctgc
      421 gtaccaaacg ctctatcaaa aagggcgagt tcgttaatga atatgtcggt gagctgattg
      481 acgaagaaga gtgccgtctg cgtatcaaac gtgctcacga gaacagcgtg acgaattttt
      541 acatgctgac tgtaacaaaa gatcgcatta tcgacgcggg cccgaagggt aactattcac
      601 gttttatgaa tcactcctgt aacccgaatt gcgaaaccca aaaatggacg gttaacgggg
      661 atgtgcgcgt cggcctgttt gccctgtgcg acattcctgc tggtatggag ctgaccttta
      721 attataacct ggattgcctg gggaatggcc gtaccgaatg tcactgcggt gctgataact
      781 gttcgggctt cctgggatga tgacgaagct tgcggccgca ctcgagcacc accaccacca
      841 ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      901 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      961 gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg
//
