LOCUS       sequence-126227-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 4Z02
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(457..477)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(490..507)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             517..539
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    554..578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(578..597)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(579..597)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(681..700)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        906..983
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(1003..1022)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttcgtcatc actccccgtg gacgcggctc aggtggttca tggcgcggtc
      121 aaaagcgatc cgcacggcct tccggatgct ggaattcctc ccttccatca tctttaactt
      181 gtctatagtt tcgtcaatac caaggggaac cattttggac ttaggaagcc actgccaact
      241 tctcttatta tcaaaaaaga gaacgaggaa cagcttctca tcagacttgg tctgcatgtg
      301 ctccccaatc ttcagcacgt ccaggggtgg ggccgggatg gtgacgccgt tgtggtggcc
      361 aggcacacgg ggcatcttgg ggtcgatgat cagtgccggg taggaggggt agccgctgca
      421 cttggcccac accaccttca gaggctccag caccgagccc tggaaataca agttttctct
      481 gccgctgctg tgatgatgat gatgatgcat ggtatatctc cttcttaaag ttaaacaaaa
      541 ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt
      601 tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
      661 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
      721 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
      781 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
      841 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaga
      901 tcccggacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag
      961 agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg
     1021 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga
     1081 aaacgcggga aa
//
