LOCUS       sequence-126228-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 4Z02
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             503..520
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(573..591)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      587..634
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(758..777)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     968..989
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgcat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggctc ggtgctggag cctctgaagg
      121 tggtgtgggc caagtgcagc ggctacccct cctacccggc actgatcatc gaccccaaga
      181 tgccccgtgt gcctggccac cacaacggcg tcaccatccc ggccccaccc ctggacgtgc
      241 tgaagattgg ggagcacatg cagaccaagt ctgatgagaa gctgttcctc gttctctttt
      301 ttgataataa gagaagttgg cagtggcttc ctaagtccaa aatggttccc cttggtattg
      361 acgaaactat agacaagtta aagatgatgg aagggaggaa ttccagcatc cggaaggccg
      421 tgcggatcgc ttttgaccgc gccatgaacc acctgagccg cgtccacggg gagtgatgac
      481 gaagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
      541 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      601 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
      661 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      721 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      781 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      841 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      901 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      961 tttaatagtg gactcttgtt ccaaactgga acaacact
//
