LOCUS       sequence-126239-        1146 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1146)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 4Z31
FEATURES             Location/Qualifiers
     source          1..1146
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1036..1056)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(1069..1086)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             1096..1118
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttcgtcatc actgaggggt ctcatggcct tttctactat aattttgtat
      121 gaagtccaca aggccatgaa ctactgtttt aacagctacc attgcatttt ccagctgctc
      181 ccaagttggt gaaacagcgt ctggattagg gtctatgttt gcaagaagct ctaaatgagg
      241 cctcagtcta tttaagttag ctgggtcacc tgttcgttgc aaaacaattg tcaattcctg
      301 gacactcttt gcaaatgact ctggagactg tagcttatca atgatagact gcatgtgtga
      361 tttatgagcc aaatcaccat acaaaagaga ggaccactgt tcaggtgaaa tacggagtcc
      421 tgcttccatg gcaatatgaa caatttgggc atcatgttct ctgcgtaatg cttcataact
      481 ccgaaattcc tccttcagct gcattaggga agagtcttca tctcttttgg taaccttaaa
      541 acaagaagct cgatacagta gttgcacaac atgaccaata cttgtttttg atgcctgagg
      601 aaatcttggt tctagtctct gcacaacaaa aagtaccaga actttccttg agagggcaga
      661 accatcttct aatgccagta acaccagctt caaggcctct tcttgcatag ctggccctaa
      721 aaactggcat cctcgagccc tgacagcggc ccatagattg gcagacaact gctgagggtt
      781 ctggtgctgt aatatcagtt ctgttacagt tctttctcca agggaacgag ctgctcgcat
      841 ggctcttaca cgaccttctt cctccaccag ttgacaattt acaagtgtca ccagtttcct
      901 ttgcattgga cggctcagtg cactctggtt caagctagct acacctttac ctccacttag
      961 tggttttaag tagagtgcca aatcctcaac gcatttcttt gcaacctcat agtgtttatt
     1021 ctcacctaga ttactgccct ggaaatacaa gttttctctg ccgctgctgt gatgatgatg
     1081 atgatgcatg gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt
     1141 tatccg
//
