LOCUS       sequence-126241-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 4Z31
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg catcatcatc
       61 atcatcacag cagcggcaga gaaaacttgt atttccaggg cagtaatcta ggtgagaata
      121 aacactatga ggttgcaaag aaatgcgttg aggatttggc actctactta aaaccactaa
      181 gtggaggtaa aggtgtagct agcttgaacc agagtgcact gagccgtcca atgcaaagga
      241 aactggtgac acttgtaaat tgtcaactgg tggaggaaga aggtcgtgta agagccatgc
      301 gagcagctcg ttcccttgga gaaagaactg taacagaact gatattacag caccagaacc
      361 ctcagcagtt gtctgccaat ctatgggccg ctgtcagggc tcgaggatgc cagtttttag
      421 ggccagctat gcaagaagag gccttgaagc tggtgttact ggcattagaa gatggttctg
      481 ccctctcaag gaaagttctg gtactttttg ttgtgcagag actagaacca agatttcctc
      541 aggcatcaaa aacaagtatt ggtcatgttg tgcaactact gtatcgagct tcttgtttta
      601 aggttaccaa aagagatgaa gactcttccc taatgcagct gaaggaggaa tttcggagtt
      661 atgaagcatt acgcagagaa catgatgccc aaattgttca tattgccatg gaagcaggac
      721 tccgtatttc acctgaacag tggtcctctc ttttgtatgg tgatttggct cataaatcac
      781 acatgcagtc tatcattgat aagctacagt ctccagagtc atttgcaaag agtgtccagg
      841 aattgacaat tgttttgcaa cgaacaggtg acccagctaa cttaaataga ctgaggcctc
      901 atttagagct tcttgcaaac atagacccta atccagacgc tgtttcacca acttgggagc
      961 agctggaaaa tgcaatggta gctgttaaaa cagtagttca tggccttgtg gacttcatac
     1021 aaaatt
//
