LOCUS       sequence-126243-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration of 2GFP tag at Atg1 C terminus, use auxotrophic marker 
            URA3(Candida albicans).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: LD201
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..725)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(12..725)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ggcgcgcctt atttatataa ttcatccata ccatgggtaa taccagcagc agtaacaaat
       61 tctaacaaga ccatgtggtc tctcttttcg tttggatctt tggataaggc agattgagtg
      121 gataagtaat ggttgtctgg taacaagact ggaccatcac caattggagt attttgttga
      181 taatggtcag ctaattgaac agaaccatct tcaatgttgt gtctaatttt gaagttaact
      241 ttgataccat tcttttgttt gtcagccatg atgtaaacat tgtgagagtt atagttgtat
      301 tccaatttgt gacctaaaat gttaccatct tctttaaaat caataccttt taattcgatt
      361 ctattaacta aggtatcacc ttcaaacttg acttcagctc tggtcttgta gttaccgtca
      421 tctttgaaaa aaatagttct ttcttgaaca taaccttctg gcatggcaga cttgaaaaag
      481 tcatgttgtt tcatatgatc tgggtatcta gaaaaacatt gaacaccata agttaaagta
      541 gtgactaagg ttggccatgg aactggcaat ttaccagtag tacaaataaa ttttaaggtc
      601 aatttaccgt aagtagcatc accttcacct tcaccggaga cagaaaattt gtgaccatta
      661 acatcaccat ctaattcaac caaaattggg acaacaccag tgaataattc ttcaccttta
      721 gacatgaatg gcttctctag atcacgcgtt ggaactggaa ttttgtacaa ttcatcaata
      781 ccatgggtaa taccagcagc agtaacaaat tctaacaaga ccatgtggtc tctcttttcg
      841 tttggatctt
//
