LOCUS       sequence-126247-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration of 2GFP tag at Atg21 C terminus, use auxotrophic marker 
            URA3(Candida albicans).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: LD221
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..780)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(67..780)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 taacttattt aataataaaa atcataaatc ataagaaatt cgcttattta gaagtggcgc
       61 gccttattta tataattcat ccataccatg ggtaatacca gcagcagtaa caaattctaa
      121 caagaccatg tggtctctct tttcgtttgg atctttggat aaggcagatt gagtggataa
      181 gtaatggttg tctggtaaca agactggacc atcaccaatt ggagtatttt gttgataatg
      241 gtcagctaat tgaacagaac catcttcaat gttgtgtcta attttgaagt taactttgat
      301 accattcttt tgtttgtcag ccatgatgta aacattgtga gagttatagt tgtattccaa
      361 tttgtgacct aaaatgttac catcttcttt aaaatcaata ccttttaatt cgattctatt
      421 aactaaggta tcaccttcaa acttgacttc agctctggtc ttgtagttac cgtcatcttt
      481 gaaaaaaata gttctttctt gaacataacc ttctggcatg gcagacttga aaaagtcatg
      541 ttgtttcata tgatctgggt atctagaaaa acattgaaca ccataagtta aagtagtgac
      601 taaggttggc catggaactg gcaatttacc agtagtacaa ataaatttta aggtcaattt
      661 accgtaagta gcatcacctt caccttcacc ggagacagaa aatttgtgac cattaacatc
      721 accatctaat tcaaccaaaa ttgggacaac accagtgaat aattcttcac ctttagacat
      781 gaatggcttc tctagatcac gcgttggaac tggaattttg tacaattcat caataccatg
      841 ggtaatacca gcagcagtaa caaattctaa caagaccatg tggtctctct tttcgttt
//
