LOCUS       sequence-126249-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Cujec TP, Okamoto H, Fujinaga K, Meyer J, Chamberlin H, Morgan DO, 
            Peterlin BM
  TITLE     The HIV transactivator TAT binds to the CDK-activating kinase and 
            activates the phosphorylation of the carboxy-terminal domain of RNA 
            polymerase II.
  JOURNAL   Genes Dev. 1997 Oct 15. 11(20):2645-57.
  PUBMED    9334327
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            1997 Oct 15. 11(20):2645-57."
COMMENT     Sequence Label: pcDNA3 Tat HA (P#29)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             413..439
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(506..524)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(507..524)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(544..561)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    550..774
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccgccacca tggagccagt
      121 agatcctaat ctagagccct ggaagcatcc aggaagtcag cctaggactg cttgtaacaa
      181 ttgctattgt aaaaagtgtt gctttcattg ctacgcgtgt ttcacaagaa aaggcttagg
      241 catctcctat ggcaggaaga agcggagaca gcgacgaaga gctcctcagg acagtcagac
      301 tcatcaagct tctctatcaa agcaacccgc ctcccagtcc cgaggggacc cgacagggcc
      361 cacggaatcg aagaagaagg tggagagaga gacagagaca gatccgttcg attacccata
      421 cgatgttcca gattacgctg cctgagaatt ctgcagatat ccatcacact ggcggccgct
      481 cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
      541 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
      601 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
      661 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
      721 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
      781 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
      841 taagcgcggc gg
//
