LOCUS       sequence-126256-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  complementation of yeast auxotrophic marker URA3(Kluyveromyces 
            lactis).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: BS-Ura3Kl
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    46..79
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gatatcaagc ttatcgatgt acgctgcagg tcgacaaccc ttaatataac ttcgtataat
       61 gtatgctata cgaagttatt aggtctagag atcccaatac aacagatcac gtgatctttt
      121 tgtaagatga agttgaagta gtgttgcacc gtgccaatgc aggtggctat tagattaaat
      181 atgtgatttg ttctattaag tttcctgtat aattaatggg gagcgctgat tctcttttgg
      241 tacgcttccc atccagcatt tctgtatctt tcaccttcaa ccttaggatc tctacccttg
      301 gcgaaaagtc ctctgccaac aatgatgata tctgatccac cacttacaac ttcgtcgacg
      361 gttctgtact gctgacccaa tgcatcgcct ttgtcgtcta aacctacacc tggggtcatg
      421 attagccaat caaacccttc ttctcttcct cccatatcgt tctgagcaat gaacccaata
      481 acgaaatctt tatcactctt tgcaatatca acggtaccct tagtatattc accgtgtgct
      541 agagaaccct tggaagacaa ttcagcaagc atcaataatc cccttggttc tttggtgacc
      601 tcttgcgcac cttgtttcaa gccagcaaca ataccagcac cagtaacccc gtgggcgttg
      661 gtgatatcag accattctgc gatacggtaa acgcccgatg tatattgtaa tttgactgtg
      721 ttaccgatat cggcgaattt tctgtcctca aatatcaaga acttgtattt ctctgccaat
      781 gctttcaatg gaacgacagt accctcataa ctgaaatcat ccaagatatc aacgtgtgtt
      841 ttcaaaaggc aaatgtatgg acccaacgtt tcaacaagtt tcaatagctc atcagtcgaa
      901 cgaacgtcaa gagaagcaca caaattggtc ttcttttcat ccattaaacg taaaagtttc
      961 gatgcaaccg gacttgcatg agtctcagct ctactggtat atga
//
