LOCUS       sequence-126259-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting NanoLuc (NL1), constiutive 
            NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM731
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttcagctttc gttaaattat caaacttacg ttgagtgatt aacttggcgt ttagaagttg
       61 tctccaatag tttttcatct ttttgactac ttcttcactt ggaacgttat ccgatttacc
      121 acgattttta tcagaacgcg ttaagacctt attgtctatt gaatcgtctt taaggaaact
      181 ttgtggaaca atggcatcga catcataatc acttaaacga ttaatatcta attcttggtc
      241 cacatacatg tctcttccat tttggagata atagagatag agcttttcat tttgcaattg
      301 agtattttca acaggatgct ctttaagaat ctgacttcct aattctttga taccttcttc
      361 gattcgtttc atacgctctc gcgaattttt ctggcccttt tgagttgtct gattttcacg
      421 tgccatttca ataacgatat tttctggctt atgccgcccc attactttga ccaattcatc
      481 aacaactttt acagtctgta aaataccttt tttaatagca gggctaccag ctaaatttgc
      541 aatatgttca tgtaaactat cgccttgtcc agacacttgt gctttttgaa tgtcttcttt
      601 aaatgtcaaa ctatcatcat ggatcagctg cataaaattg cgattggcaa aaccatctga
      661 tttcaaaaaa tctaatattg ttttgccaga ttgcttatcc ctaataccat taatcaattt
      721 tcgagacaaa cgtccccaac cagtataacg gcgacgttta agctgtttca tcaccttatc
      781 atcaaagagg tgagcatatg t
//
