LOCUS       sequence-126260-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5C4Q
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(549..567)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      563..610
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(785..790,791..807,808..813))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(840..858)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        926..1030
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catgcatcat catcatcatc
       61 acagcagcgg cagagaaaac ttgtatttcc agggcatgga cgtcagcaag cggccacgcg
      121 aggaattcca caaggagcag tgtctctcct tcgtgaagaa gctttgggcg gccgacacgc
      181 tcgccatgtt tcactatccg gtgagcgcca ccgaggtgcc cggctactac gacgtcgtag
      241 atacacctat ggacctgtcc acgattcgga agaacatcga gcagggcaag tacagaacgg
      301 acactgaggt cgagaatgac gtggtgctca tgctgtcgaa cgctctggac tttaacgaga
      361 aaggctcgca atggcatgat ttggcgaagc agctcaagaa gcggtacctg acccttgcgc
      421 aggagtcggg gctgtccttc gacgcagacc aggcgttcat ccctacgaaa tgatgacgaa
      481 gcttgcggcc gcactcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      541 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      601 gggttttttg ctgaaaggag gaactatatc cggatatccc gcaagaggcc cggcagtacc
      661 ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc
      721 gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta
      781 ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg
      841 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      901 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      961 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1021 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1081 ttgccttcct gtttttg
//
