LOCUS       sequence-126261-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5C4Q
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(436..456)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(469..486)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             496..518
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    533..557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(557..576)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(558..576)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(660..679)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat cctcgagtgc ggccgcaagc ttcgtcatca
       61 tttcgtaggg atgaacgcct ggtctgcgtc gaaggacagc cccgactcct gcgcaagggt
      121 caggtaccgc ttcttgagct gcttcgccaa atcatgccat tgcgagcctt tctcgttaaa
      181 gtccagagcg ttcgacagca tgagcaccac gtcattctcg acctcagtgt ccgttctgta
      241 cttgccctgc tcgatgttct tccgaatcgt ggacaggtcc ataggtgtat ctacgacgtc
      301 gtagtagccg ggcacctcgg tggcgctcac cggatagtga aacatggcga gcgtgtcggc
      361 cgcccaaagc ttcttcacga aggagagaca ctgctccttg tggaattcct cgcgtggccg
      421 cttgctgacg tccatgccct ggaaatacaa gttttctctg ccgctgctgt gatgatgatg
      481 atgatgcatg gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt
      541 tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat
      601 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
      661 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
      721 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
      781 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
      841 cttcctaatg caggagtcgc ataagggaga gcgtcagatc ccggacacca tcgaatggcg
      901 caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtg
//
