LOCUS       sequence-126271-         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting PcfiA (PR2), constiutive 
            NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM750
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cacgttcagc tttcgttaaa ttatcaaact tacgttgagt gattaacttg gcgtttagaa
       61 gttgtctcca atagtttttc atctttttga ctacttcttc acttggaacg ttatccgatt
      121 taccacgatt tttatcagaa cgcgttaaga ccttattgtc tattgaatcg tctttaagga
      181 aactttgtgg aacaatggca tcgacatcat aatcacttaa acgattaata tctaattctt
      241 ggtccacata catgtctctt ccattttgga gataatagag atagagcttt tcattttgca
      301 attgagtatt ttcaacagga tgctctttaa gaatctgact tcctaattct ttgatacctt
      361 cttcgattcg tttcatacgc tctcgcgaat ttttctggcc cttttgagtt gtctgatttt
      421 cacgtgccat ttcaataacg atattttctg gcttatgccg ccccattact ttgaccaatt
      481 catcaacaac ttttacagtc tgtaaaatac cttttttaat agcagggcta ccagctaaat
      541 ttgcaatatg ttcatgtaaa ctatcgcctt gtccagacac ttgtgctttt tgaatgtctt
      601 ctttaaatgt caaactatca tcatggatca gctgcataaa attgcgattg gcaaaaccat
      661 ctgatttcaa aaaatctaat attgttttgc cagat
//
