LOCUS       sequence-126273-         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting PcfiA (PR1), constiutive 
            NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM763
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttaaattatc aaacttacgt tgagtgatta acttggcgtt tagaagttgt ctccaatagt
       61 ttttcatctt tttgactact tcttcacttg gaacgttatc cgatttacca cgatttttat
      121 cagaacgcgt taagacctta ttgtctattg aatcgtcttt aaggaaactt tgtggaacaa
      181 tggcatcgac atcataatca cttaaacgat taatatctaa ttcttggtcc acatacatgt
      241 ctcttccatt ttggagataa tagagataga gcttttcatt ttgcaattga gtattttcaa
      301 caggatgctc tttaagaatc tgacttccta attctttgat accttcttcg attcgtttca
      361 tacgctctcg cgaatttttc tggccctttt gagttgtctg attttcacgt gccatttcaa
      421 taacgatatt ttctggctta tgccgcccca ttactttgac caattcatca acaactttta
      481 cagtctgtaa aatacctttt ttaatagcag ggctaccagc taaatttgca atatgttcat
      541 gtaaactatc gccttgtcca gacacttgtg ctttttgaat gtcttcttta aatgtcaaac
      601 tatcatcatg gatcagctgc ataaaattgc gattggcaaa accatctgat ttcaaa
//
