LOCUS       sequence-126274-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Twister plasmid to swap inserts from an omega1 or omega1R vector to 
            any alpha level vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1omega2_SF (GB0466)
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    29..53
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    243..265
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     rep_origin      392..827
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 atctcaaaca aacacataca gcgacttagt ttacccgcca atatatcctg tcagatatcg
       61 gtctcaagcg aaatgattga tgaagaacaa tggtggatga agaacaaaga aggagggagc
      121 ttttgttcaa gatgaacaaa gaacaatagt ggatgaagaa caaagtgaaa aaaataaaaa
      181 aaaatgtatg gttaaataaa gagtaaagtt accattgaga ctcctgactg agaccgatat
      241 cgttacacca caatatatcc tgccaagatc taattccggg gatcggaaat ccagaagccc
      301 gagaggttgc cgcctttcgg gctttttctt tttcaaaaaa aaaaatttat aaaacgatct
      361 gttgcggccg gccgccgggt tgtgggcaaa ggcgctcgac ggtgggcaac cgcttgcggt
      421 tgtccacggg cggagccggt gcgcgtagcg cattgtccac aagccaaggg cgaccaataa
      481 ttgatatata tattcataat tgaaaagcta attgaacata ctacttgctg taactacttg
      541 ccggagcgag gggtgtttgc aagctgttga tctgaaaggg ctattagcgt tctcacgtgc
      601 ctttttgatt agcgatttca cgtgacctta ttagcgattt cacgtactcc gattagcgat
      661 ttcacgtacc ctgattagcg atttcacgtg gatagttttt ggagcgggcc ggaaagcccc
      721 gtgaatcaag gctttgcggg gcattagcgg tttcacgtgg ataactaccc tctatccaca
      781 ggcttccggg gataaaaaag cccgctcgac ggcgggctgt tggatgggga tcgcctgaat
      841 cgccccatca tccagccaga aagtgaggga
//
