LOCUS       sequence-126276-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5C8G
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(588..606)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      602..649
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(824..829,830..846,847..852))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(879..897)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg catcatcatc
       61 atcatcacag cagcggcaga gaaaacttgt atttccaggg ccatccacta ccgcttccaa
      121 agaacaagca cgtattccat agtgaccaga ggctggcacc agagattcgc gacttgtacg
      181 actgcttata caaactttat gcagaggaga gcgcctcgga atatttcagg gaacctgtcg
      241 atgcactgag ggtgggggcg tggaactatt acagtgtcat cacggaacca atgtcgctgc
      301 gcacggtgct tgactacatt gtccagggcg ggcgctactc tcacgtcgaa caaataatga
      361 atgacgtcga actgatttgg aaaaactgtg agaggtataa cggggccgaa tcgcatcttg
      421 ccgccgacgc acgcaggtgt agggcgatac ttgagaagca ccgggaacgg cttgctgacg
      481 agcagactgc acccgctgca gaggtggatt gatgacgaag cttgcggccg cactcgagga
      541 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      601 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      661 aactatatcc ggatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct
      721 acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac
      781 ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg
      841 ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt
      901 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
      961 tgtgcgcgga acccctattt g
//
