LOCUS       sequence-126277-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5C8G
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(463..483)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(496..513)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             523..545
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    560..584
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(584..603)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(585..603)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(687..706)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        912..989
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 ttcgggcttt gttagcagcc ggatcctcga gtgcggccgc aagcttcgtc atcaatccac
       61 ctctgcagcg ggtgcagtct gctcgtcagc aagccgttcc cggtgcttct caagtatcgc
      121 cctacacctg cgtgcgtcgg cggcaagatg cgattcggcc ccgttatacc tctcacagtt
      181 tttccaaatc agttcgacgt cattcattat ttgttcgacg tgagagtagc gcccgccctg
      241 gacaatgtag tcaagcaccg tgcgcagcga cattggttcc gtgatgacac tgtaatagtt
      301 ccacgccccc accctcagtg catcgacagg ttccctgaaa tattccgagg cgctctcctc
      361 tgcataaagt ttgtataagc agtcgtacaa gtcgcgaatc tctggtgcca gcctctggtc
      421 actatggaat acgtgcttgt tctttggaag cggtagtgga tggccctgga aatacaagtt
      481 ttctctgccg ctgctgtgat gatgatgatg atgcatggta tatctccttc ttaaagttaa
      541 acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata gtgagtcgta
      601 ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac
      661 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      721 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      781 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
      841 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
      901 tcgagatccc ggacaccatc gaatggcgca aaacctttcg cggtatggca tgatagcgcc
      961 cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgt
//
