LOCUS       sequence-126281-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), 
            constiutive NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM764
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctttcgttaa attatcaaac ttacgttgag tgattaactt ggcgtttaga agttgtctcc
       61 aatagttttt catctttttg actacttctt cacttggaac gttatccgat ttaccacgat
      121 ttttatcaga acgcgttaag accttattgt ctattgaatc gtctttaagg aaactttgtg
      181 gaacaatggc atcgacatca taatcactta aacgattaat atctaattct tggtccacat
      241 acatgtctct tccattttgg agataataga gatagagctt ttcattttgc aattgagtat
      301 tttcaacagg atgctcttta agaatctgac ttcctaattc tttgatacct tcttcgattc
      361 gtttcatacg ctctcgcgaa tttttctggc ccttttgagt tgtctgattt tcacgtgcca
      421 tttcaataac gatattttct ggcttatgcc gccccattac tttgaccaat tcatcaacaa
      481 cttttacagt ctgtaaaata ccttttttaa tagcagggct accagctaaa tttgcaatat
      541 gttcatgtaa actatcgcct tgtccagaca cttgtgcttt ttgaatgtct tctttaaatg
      601 tcaaactatc atcatggatc agctgcataa aattgcgatt ggcaaaacca tctgatttca
      661 aaaaatctaa tattgttttg ccagattgct tatccctaat accattaatc aattttcgag
      721 acaaacgtcc ccaaccagta taacggcgac gtttaagctg tttcatcacc ttatcatc
//
