LOCUS       sequence-126288-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TCR beta chain genomic transgenic construct for creating OT-II mice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Barnden MJ, Allison J, Heath WR, Carbone FR
  TITLE     Defective TCR expression in transgenic mice constructed using 
            cDNA-based alpha- and beta-chain genes under the control of 
            heterologous regulatory elements.
  JOURNAL   Immunol Cell Biol. 1998 Feb;76(1):34-40.
  PUBMED    9553774
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunol 
            Cell Biol."; date: "1998-02"; volume: "76(1)"; pages: "34-40"
COMMENT     Sequence Label: p3A9cbetaTCR.20beta
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     57..73
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     57..73
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 aattgtaata cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta
       61 gaactagtgg atctgcatgg tgcatactga aggcttctgt ttcctgtgtg acttctaaca
      121 gatgtaagat agtttagatg gggggcttga gatgagatat atgggttccc ctttagagac
      181 cctcctcttg gttgttctca ctgtcacggt gatagctaga ggctgaggta gaaagggctg
      241 catgagaagg gtttgaagac aggatgtggc aagtgtggtt cccaaaatgc tcagaatgtg
      301 agtgagtggg ggagcattca caggcacccc agatgtagga cctggtaaat gtcaaaccac
      361 atcctgttgt gactcctgga gatggagcta tggcaggtgg ccattcttac ttggtgctga
      421 tgttacttac agataaacag ccagacctgg ggtgagatgc ttccacccct tcccccattg
      481 tcttcaggaa ccgaaaagtc ccaggaatgc atctcttcac ttgctgatgt ttgatgaaaa
      541 acagttcttc ttaagattat tttttttatc atgttcctat atctctttgc tggaatcggt
      601 cagggaaaca agtattaagg gtcctactgt gcctggttaa ctatgggact caagatcagc
      661 actgtccctt tgcaggtcag atgccttgta agatatatca caatgtgtgt gtctggagct
      721 gggaaggact tagatttccc cacttaccat tctgtcagtg ctacaaggcc tgc
//
