LOCUS       sequence-126291-        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5C0M
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(582..593)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             668..685
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(738..756)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      752..799
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(923..942)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg catcatcatc
       61 atcatcacag cagcggcaga gaaaacttgt atttccaggg ccgcagaggg gtgctgatga
      121 ccctgctgca gcagtcggcc atgaccctgc ccctgtggat cgggaagcct ggtgacaagc
      181 ccccacccct ctgtggggcc atccctgcct caggagacta cgtggccaga cctggagaca
      241 aggtggctgc ccgggtgaag gccgtggatg gggacgagca gtggatcctg gccgaggtgg
      301 tcagttacag ccatgccacc aacaagtatg aggtagatga catcgatgaa gaaggcaaag
      361 agagacacac cctgagccgg cgccgtgtca tcccgctgcc ccagtggaag gccaacccgg
      421 agacggaccc tgaggccttg ttccagaagg agcagctcgt gctggccctg tatccccaga
      481 ctacctgctt ctaccgcgcc ctgatccatg cgcccccaca gcggccccag gatgactact
      541 cggtcctgtt tgaagacacc tcctatgcag atggctattc ccctcccctc aatgtggctc
      601 agagatacgt ggtggcttgt aaggaaccca agaaaaagtg atgacgaagc ttgcggccgc
      661 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      721 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      781 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
      841 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      901 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      961 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     1021 ttacggcacc tcgaccc
//
