LOCUS       sequence-126292-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5C0M
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..138
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(619..639)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(652..669)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             679..701
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    716..740
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(740..759)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(741..759)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(843..862)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttcgtcatc actttttctt gggttcctta caagccacca cgtatctctg
      121 agccacattg aggggagggg aatagccatc tgcataggag gtgtcttcaa acaggaccga
      181 gtagtcatcc tggggccgct gtgggggcgc atggatcagg gcgcggtaga agcaggtagt
      241 ctggggatac agggccagca cgagctgctc cttctggaac aaggcctcag ggtccgtctc
      301 cgggttggcc ttccactggg gcagcgggat gacacggcgc cggctcaggg tgtgtctctc
      361 tttgccttct tcatcgatgt catctacctc atacttgttg gtggcatggc tgtaactgac
      421 cacctcggcc aggatccact gctcgtcccc atccacggcc ttcacccggg cagccacctt
      481 gtctccaggt ctggccacgt agtctcctga ggcagggatg gccccacaga ggggtggggg
      541 cttgtcacca ggcttcccga tccacagggg cagggtcatg gccgactgct gcagcagggt
      601 catcagcacc cctctgcggc cctggaaata caagttttct ctgccgctgc tgtgatgatg
      661 atgatgatgc atggtatatc tccttcttaa agttaaacaa aattatttct agaggggaat
      721 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc
      781 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
      841 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
      901 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
      961 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     1021 ctgcttccta atgcaggagt cgcataaggg agagcgt
//
