LOCUS       sequence-126298-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5C7J
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catgcatcat catcatcatc
       61 acagcagcgg cagagaaaac ttgtatttcc agggcaggga ttacaaaaga aagtatgagt
      121 tcttccgaag aaagttgaag aagcagaatg acattccaaa caaatttgaa atgaaacttc
      181 gccgagcaac tgttcttgaa gactcttacc ggagaattat gggtgtcaag agagcagact
      241 tcctgaaggc tcgactgtgg attgagtttg atggtgaaaa gggattggat tatggaggag
      301 ttgccagaga atggttcttc ctgatctcaa aggaaatgtt taacccttat tatgggttgt
      361 ttgaatattc tgctacggac aattataccc tacagataaa tccaaactct ggattgtgta
      421 acgaagatca cctctcttac ttcaagttta ttggtcgggt agctggaatg gcagtttatc
      481 atggcaaact gttggatggt tttttcatcc gcccatttta caagatgatg cttcacaaac
      541 caataaccct tcatgatatg gaatctgtgg atagtgaata ttacaattcc ctaagatgga
      601 ttcttgaaaa tgacccaaca gaattggacc tcaggtttat catagatgaa gaactttttg
      661 gacagacaca tcaacatgag ctgaaaaatg gtggatcaga aatagttgtc accaataaga
      721 acaaaaagga atatatttat cttgtaatac aatggcgatt tgtaaaccga atccagaagc
      781 aaatggctgc ttttaaagag ggattctttg aactaatacc acaggatctc atcaaaattt
      841 ttgatgaaaa tgaactagag cttcttatgt gtggactggg agatgttgat gtgaatgact
      901 ggagggaaca tacaaagtat aaaaatggct acagtgcaaa tcatcaggtt atacagtggt
      961 tttggaaggc tgttttaatg atggattcag aaaaaagaat aagattactt cagttt
//
