LOCUS       sequence-126305-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5C7M (ITCH)
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg catcatcatc
       61 atcatcacag cagcggcaga gaaaacttgt atttccaggg ctatgttcgg gacttcaaag
      121 caaaggttca gtatttccgg ttctggtgtc agcaactggc catgccacag cacataaaga
      181 ttacagtgac aagaaaaaca ttgtttgagg attcctttca acagataatg agcttcagtc
      241 cccaagatct gcgaagacgt ttgtgggtga tttttccagg agaagaaggt ttagattatg
      301 gaggtgtagc aagagaatgg ttctttcttt tgtcacatga agtgtcgaac ccaatgtatt
      361 gcctgtttga atatgcaggg aaggataact actgcttgca gataaacccc gcttcttaca
      421 tcaatccaga tcacctgaaa tattttcgtt ttattggcag atttattgcc atggctctgt
      481 tccatgggaa attcatagac acgggttttt ctttaccatt ctataagcgt atcttgaaca
      541 aaccagttgg actcaaggat ttagaatcta ttgatccaga attttacaat tctctcatct
      601 gggttaagga aaacaatatt gaggaatgtg atttggaaat gtacttctcc gttgacaaag
      661 aaattctagg tgaaattaag agtcatgatc tgaaacctaa tggtggcaat attcttgtaa
      721 cagaagaaaa taaagaggaa tacatcagaa tggtagctga gtggaggttg tctcgaggtg
      781 ttgaagaaca gacacaagct ttctttgaag gctttaatga aattcttccc cagcaatatt
      841 tgcaatactt tgatgcaaag gaattagagg tccttttatg tggaatgcaa gagattgatt
      901 tgaatgactg gcaaagacat gccatctacc gtcgttatgc aaggaccagc aaacaaatca
      961 tgtggttttg gcagtttgtt aaagaaattg ataatgagaa gagaatgaga cttctgcagt
     1021 ttgttactgg aacctgccga ttgccagtag gaggatttgc tg
//
