LOCUS       sequence-126313-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5C7M (UBC)
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             282..299
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             349..366
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(419..437)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      433..480
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      517..973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(604..623)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     814..835
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaacct taagaaggag atatactatg catattttag
       61 tgaaaaccct tagggggaag accatcaccc tcgaggttga accctcggat acgatagaaa
      121 atgtaaaggc caagatccag gataaggaag gaattcctcc tgatcagcag agactgttgt
      181 ttggcggcaa caagctggaa gatggacgta ctttgtctga ctacaatatt caaaaggaat
      241 ctaatcttta tcttctgctg agacgtcttg gtagtaagtt tcaccatcat caccaccatt
      301 gaggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac
      361 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      421 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      481 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
      541 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      601 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      661 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      721 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      781 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      841 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
      901 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
      961 cgctttacaa ttt
//
