LOCUS       sequence-126314-        1093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1093)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5C7M (UBC)
FEATURES             Location/Qualifiers
     source          1..1093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(100..117)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    395..419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(419..438)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(420..438)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(522..541)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        747..824
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(844..863)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ttctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg
       61 gccgcaagct tgtcgacgga gctcgaattc ggatcctcaa tggtggtgat gatggtgaaa
      121 cttactacca agacgtctca gcagaagata aagattagat tccttttgaa tattgtagtc
      181 agacaaagta cgtccatctt ccagcttgtt gccgccaaac aacagtctct gctgatcagg
      241 aggaattcct tccttatcct ggatcttggc ctttacattt tctatcgtat ccgagggttc
      301 aacctcgagg gtgatggtct tccccctaag ggttttcact aaaatatgca tagtatatct
      361 ccttcttaag gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc
      421 ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat
      481 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
      541 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
      601 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
      661 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
      721 gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta
      781 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
      841 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
      901 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
      961 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
     1021 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
     1081 gcgccgatca act
//
