LOCUS       sequence-126321-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5C8H
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(442..462)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(475..492)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             502..524
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    539..563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(563..582)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(564..582)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(666..685)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        891..968
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(988..1007)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttcgtcatc aagcctcctc ttcttccttt tccaagaaat cagtcaatgt
      121 tccattatca acaggaatta ataaatactc tactccatca gttcccttct ttgttcttat
      181 aagcttgtgg tccctaaatt cagttaactg ggcccggagt gtcagatcac tattgacgag
      241 gaatgcctcc cgacactgct ggtaaaaatc ttgaaaagaa aggccaatgt aagaagggtt
      301 atcctggttg tccagctggt attttattag tagcctgaaa attccccttg cattaggggt
      361 aaggcttcgt aagacatgag taagggagct aagtggcagg gatccagact gctttaccag
      421 aagagagttc tcataggagg tgccctggaa atacaagttt tctctgccgc tgctgtgatg
      481 atgatgatga tgcatggtat atctccttct taaagttaaa caaaattatt tctagagggg
      541 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat
      601 ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
      661 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
      721 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
      781 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
      841 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgagatcccg gacaccatcg
      901 aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg
      961 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc
     1021 agaccgtttc ccgcgtggtg aaccaggcca gccac
//
