LOCUS       sequence-126324-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VMA intein with C-terminal portion of alpha-sarcin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: pcDNA3-FRB-VMAc-CSarcin
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..291
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             405..416
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atggcttcta ggatcctctg gcatgagatg tggcatgaag gcctggaaga ggcatctcgt
       61 ttgtactttg gggaaaggaa cgtgaaaggc atgtttgagg tgctggagcc cttgcatgct
      121 atgatggaac ggggccccca gactctgaag gaaacatcct ttaatcaggc ctatggtcga
      181 gatttaatgg aggcccaaga gtggtgcagg aagtacatga aatcagggaa tgtcaaggac
      241 ctcctccaag cctgggacct ctattatcat gtgttccgac gaatctcaaa gactagtgtt
      301 ttgcttaacg ttctttcgaa gtgtgccggc tctaaaaaat tcaggcctgc tcccgccgct
      361 gcttttgcac gtgagtgccg cggattttat ttcgagttac aagaattgaa ggaagacgat
      421 tattatggga ttactttatc tgatgattct gatcatcagt ttttgcttgc caaccaggtt
      481 gtcgtgcaca attgcaaagc tgaatctaac tctcaccacg ctccgctgtc tgacggtaaa
      541 actggctcct cttacccgca ctggttcact aacggttacg acggtgacgg taaactgccg
      601 aaaggtcgta ctccgataaa attcggtaaa tctgactgcg accgtccgcc gaaacactct
      661 aaagacggta acggtaaaac tgaccactac ctgctggagt tcccgacttt cccggacggt
      721 cacgactaca aattcgactc taaaaaaccg aaagaaaacc cgggtccggc tcgtgttatc
      781 tacacttacc cgaacaaagt tttctgcggt atcatcgctc acactaaaga aaaccagggt
      841 gaactgaaac tgtgctctca c
//
