LOCUS       sequence-126327-         195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VMA intein with C-terminal portion of alpha-sarcin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 195)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: pcDNA3-FRB-VMAc-CSarcin
FEATURES             Location/Qualifiers
     source          1..195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             108..119
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gttttgctta acgttctttc gaagtgtgcc ggctctaaaa aattcaggcc tgctcccgcc
       61 gctgcttttg cacgtgagtg ccgcggattt tatttcgagt tacaagaatt gaaggaagac
      121 gattattatg ggattacttt atctgatgat tctgatcatc agtttttgct tgccaaccag
      181 gttgtcgtgc acaat
//
