LOCUS       sequence-126332-        5064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains cytoplasmic form of ovalbumin (OVA) cDNA in pBlueRIP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Kurts C, Heath WR, Carbone FR, Allison J, Miller JF, Kosaka H
  TITLE     Constitutive class I-restricted exogenous presentation of self 
            antigens in vivo.
  JOURNAL   J Exp Med. 1996 Sep 1;184(3):923-30.
  PUBMED    9064352
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "1996-09-1"; volume: "184(3)"; pages: "923-30"
COMMENT     Sequence Label: pBlueRIP.cOVA
FEATURES             Location/Qualifiers
     source          1..5064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     672..688
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     672..688
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    685..819
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(709..728)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     763..782
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     intron          979..1044
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1629..1652)
                     /codon_start=1
                     /product="antigenic CD8+ T cell epitope from chicken 
                     ovalbumin"
                     /label=OVA peptide
                     /translation="SIINFEKL"
     primer_bind     complement(2829..2845)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2830..2846)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(2874..2892)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2913..2929)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2913..2929)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2926..2948)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2937..2953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2961..2967,2968..2985,2986..2991))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(3144..3161)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3315..3903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(4074..4865,4866..4934))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4697..4716
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4935..5039)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctccaccgcg
      661 gtggcggccg ctctagaact agtggatcca gacatgataa gatacattga tgagtttgga
      721 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
      781 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
      841 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
      901 aaatgtggta tggctgatta tgatcatgaa cagactgtga ggactgaggg gcctgaaatg
      961 agccttggga ctgtgaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1021 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1081 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc ccaagcttgg ggtcgactct
     1141 agattaagct ccagctttta tgaagtctgg atgcagcaga taacatactt ttcattttac
     1201 ccctgatact acagtgctct gggtcttgtt ggaagggaca gagtttttca gctttcttct
     1261 ttttaagggg aaacacatct gccaaagaag agaacggcgt tggttgcgat gtgcttgata
     1321 cagaagagga atggatggtc agccctaaat tcttcagaga cgcttgcagc atccactcca
     1381 gcctctgctg accctaccac ctctctgcct gcttcattga tttctgcatg tgctgcatgg
     1441 acagcttgag atatcttcag gctctctgct gaggagatgc cagacagatt ggctgaagag
     1501 ctaaacacgt cagtaatgcc catagccatt aagacagatg tgaggttgta tttttcctcc
     1561 atcttcatgc gaggtaagta cactttgatc ttcctctctt ccataacatt agaactggtc
     1621 cattcagtca gtttttcaaa gttgattata ctctcaagct gctcaaggcc tgagacttca
     1681 tcaggcaaca gcaccaacat gctcattgtc ccactggcaa atggaagctc caggatcttc
     1741 attttctcag aagccattga tgccactcta aataaaccaa tctggtacat catctgcaca
     1801 ggtttgcttt cttgctcagt cactctgaaa ggcattgctt gtgtgtcttc atccttaaat
     1861 gctttctccc acagtccttt gaagacaatg gcattaacca gaaccattgc agtttgagaa
     1921 tccacggagc ttggctgaag gacatttctg ataattccat ttgtctgact ttctacccag
     1981 gaattgatga gctccttgaa tacatcaaaa caaaattcca tgcttgctgc accgatggag
     2041 cccatggtga actctgagtt gtctttcgag gaatccaagc ttgctagagt tagggctggg
     2101 ggttactgaa tccccactag ctttatagtc caaagcaccc cccccctgac ccctggactt
     2161 tgctgtttgt cccattaagg gtggatcagc agatggccag aggggctgaa gctgcagttt
     2221 ccaaacactt gcctggtgct aggtctgcag aaagtgctca ttggacaaca acacctctac
     2281 ttagccttag ggtaattaga gtcttaatag gggctcctgg gtggcctgat gaaccagttc
     2341 acagcagggg acattgtctc agagagggtg aggccttctt tgtccttggc tgagcatttt
     2401 tccacatcat tccccaggag gctgcagttg ttgggggacc tagtaacttt gtcccttgaa
     2461 ctgttgtttc acagttccca ctgatagctg tgtcctcacc tcagctgaga ccccagctca
     2521 ccctcagagg tagaaggaaa gcagaattca agaagaaagg tcccttctct tctatctctc
     2581 cttccacaaa cccatagccc actcgggagt caactgggct gaagccccat ggtgtattct
     2641 cctgtagtat cttagatttg cacctgaagg tcagcaactc tccataagag gtgcttgctt
     2701 cagagctcag agccagctgt ctccatctct tagtacatac tctacccagc tacaaaacct
     2761 ttctcagcct ccacttggag tggttggggg atcccccggg ctgcaggaat tcgatatcaa
     2821 gctcttatcg ataccgtcga cctcgagggg gggcccggta ccagcttttg ttccctttag
     2881 tgagggttaa ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     2941 tatccgctca caattccaca caacatagga gccggaagca taaagtgtaa agcctggggt
     3001 gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3061 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3121 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3181 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3241 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3301 gcgttgctgg cgtttttcca taggctcggc ccccctgacg agcatcacaa aaatcgacgc
     3361 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt cccccctgga
     3421 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3481 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     3541 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3601 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3661 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3721 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     3781 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3841 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3901 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4141 tgactgcccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4201 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgaaaaaa agcggttagc
     4501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatgctt
     4561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5041 acatttcccc gaaaagtgcc acct
//
