LOCUS       sequence-126336-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminal portion of GFP along with VMA intein for 
            protein splicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: pcDNA3-FRB-VMAc-CGFP
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..291
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             405..416
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     762..783
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggcttcta ggatcctctg gcatgagatg tggcatgaag gcctggaaga ggcatctcgt
       61 ttgtactttg gggaaaggaa cgtgaaaggc atgtttgagg tgctggagcc cttgcatgct
      121 atgatggaac ggggccccca gactctgaag gaaacatcct ttaatcaggc ctatggtcga
      181 gatttaatgg aggcccaaga gtggtgcagg aagtacatga aatcagggaa tgtcaaggac
      241 ctcctccaag cctgggacct ctattatcat gtgttccgac gaatctcaaa gactagtgtt
      301 ttgcttaacg ttctttcgaa gtgtgccggc tctaaaaaat tcaggcctgc tcccgccgct
      361 gcttttgcac gtgagtgccg cggattttat ttcgagttac aagaattgaa ggaagacgat
      421 tattatggga ttactttatc tgatgattct gatcatcagt ttttgcttgc caaccaggtt
      481 gtcgtgcaca attgcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      541 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
      601 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      661 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
      721 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      781 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaag
//
