LOCUS       sequence-126340-         333 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminal portion of GFP along with VMA intein for 
            protein splicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: pcDNA3-FRB-VMAc-CGFP
FEATURES             Location/Qualifiers
     source          1..333
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     270..291
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
       61 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
      121 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      181 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
      241 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      301 gggatcactc tcggcatgga cgagctgtac aag
//
