LOCUS       sequence-126342-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal portion of alpha sarcin with Q27G mutation 
            along with VMA intein for protein splicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: NSarQ27G-VMAn-FKBP
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             667..987
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 gcagttactt ggacttgcct gaacgaccag aaaaacccga aaactaacaa atacgaaact
       61 aaacgtctgc tgtacaacgg gtgctttgcc aagggtacca atgttttaat ggcggatggg
      121 tctattgaat gtattgaaaa cattgaggtt ggtaataagg tcatgggtaa agatggcaga
      181 cctcgtgagg taattaaatt gcccagagga agagaaacta tgtacagcgt cgtgcagaaa
      241 agtcagcaca gagcccacaa aagtgactca agtcgtgaag tgccagaatt actcaagttt
      301 acgtgtaatg cgacccatga gttggttgtt agaacacctc gtagtgtccg ccgtttgtct
      361 cgtaccatta agggtgtcga atattttgaa gttattactt ttgagatggg ccaaaagaaa
      421 gcccccgacg gtagaattgt tgagcttgtc aaggaagttt caaagagcta cccaatatct
      481 gaggggcctg agagagccaa cgaattagta gaatcctata gaaaggcttc aaataaagct
      541 tattttgagt ggactattga ggccagagat ctttctctgt tgggttccca tgttcgtaaa
      601 gctacctacc agacttacgc tccaattctt tattctagaa acaacggcaa cgggaacggc
      661 actagaggag tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt ccccaagcgc
      721 ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa atttgattcc
      781 tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt gatccgaggc
      841 tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac tatatctcca
      901 gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc cactctcgtc
      961 ttcgatgtgg agcttctaaa actggaa
//
