LOCUS       sequence-126347-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G 
            mutation along with VMA intein for protein splicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: 3XFLAG-NSarQ27G-VMAn-FKBP
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..66
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             742..1062
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat
       61 gacaagcttg cggccgcagt tacttggact tgcctgaacg accagaaaaa cccgaaaact
      121 aacaaatacg aaactaaacg tctgctgtac aacgggtgct ttgccaaggg taccaatgtt
      181 ttaatggcgg atgggtctat tgaatgtatt gaaaacattg aggttggtaa taaggtcatg
      241 ggtaaagatg gcagacctcg tgaggtaatt aaattgccca gaggaagaga aactatgtac
      301 agcgtcgtgc agaaaagtca gcacagagcc cacaaaagtg actcaagtcg tgaagtgcca
      361 gaattactca agtttacgtg taatgcgacc catgagttgg ttgttagaac acctcgtagt
      421 gtccgccgtt tgtctcgtac cattaagggt gtcgaatatt ttgaagttat tacttttgag
      481 atgggccaaa agaaagcccc cgacggtaga attgttgagc ttgtcaagga agtttcaaag
      541 agctacccaa tatctgaggg gcctgagaga gccaacgaat tagtagaatc ctatagaaag
      601 gcttcaaata aagcttattt tgagtggact attgaggcca gagatctttc tctgttgggt
      661 tcccatgttc gtaaagctac ctaccagact tacgctccaa ttctttattc tagaaacaac
      721 ggcaacggga acggcactag aggagtgcag gtggaaacca tctccccagg agacgggcgc
      781 accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct tgaagatgga
      841 aagaaatttg attcctcccg ggacagaaac aagcccttta agtttatgct aggcaagcag
      901 gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca gagagccaaa
      961 ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat catcccacca
     1021 catgccactc tcgtcttcga tgtggagctt ctaaaactgg aa
//
