LOCUS       sequence-126350-         552 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G 
            mutation along with VMA intein for protein splicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: 3XFLAG-NSarQ27G-VMAn-FKBP
FEATURES             Location/Qualifiers
     source          1..552
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgctttgcca agggtaccaa tgttttaatg gcggatgggt ctattgaatg tattgaaaac
       61 attgaggttg gtaataaggt catgggtaaa gatggcagac ctcgtgaggt aattaaattg
      121 cccagaggaa gagaaactat gtacagcgtc gtgcagaaaa gtcagcacag agcccacaaa
      181 agtgactcaa gtcgtgaagt gccagaatta ctcaagttta cgtgtaatgc gacccatgag
      241 ttggttgtta gaacacctcg tagtgtccgc cgtttgtctc gtaccattaa gggtgtcgaa
      301 tattttgaag ttattacttt tgagatgggc caaaagaaag cccccgacgg tagaattgtt
      361 gagcttgtca aggaagtttc aaagagctac ccaatatctg aggggcctga gagagccaac
      421 gaattagtag aatcctatag aaaggcttca aataaagctt attttgagtg gactattgag
      481 gccagagatc tttctctgtt gggttcccat gttcgtaaag ctacctacca gacttacgct
      541 ccaattcttt at
//
