LOCUS       sequence-126351-         321 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G 
            mutation along with VMA intein for protein splicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: 3XFLAG-NSarQ27G-VMAn-FKBP
FEATURES             Location/Qualifiers
     source          1..321
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..321
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag
       61 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
      121 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      181 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
      241 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
      301 gtggagcttc taaaactgga a
//
