LOCUS       sequence-126352-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains membrane bound form of ovalbumin (OVA) cDNA in pBlueRIP 
            (used to generate RIP-mOVA mice).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Kurts C, Heath WR, Carbone FR, Allison J, Miller JF, Kosaka H
  TITLE     Constitutive class I-restricted exogenous presentation of self 
            antigens in vivo.
  JOURNAL   J Exp Med. 1996 Sep 1;184(3):923-30.
  PUBMED    9064352
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "1996-09-1"; volume: "184(3)"; pages: "923-30"
COMMENT     Sequence Label: pBlueRIP.TfrOVA
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     54..70
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     54..70
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    67..201
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(91..110)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     145..164
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     intron          361..426
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 tgtaatacga ctcactatag ggcgaattgg agctccaccg cggtggcggc cgctctagaa
       61 ctagtggatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
      121 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
      181 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
      241 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
      301 tatgatcatg aacagactgt gaggactgag gggcctgaaa tgagccttgg gactgtgaat
      361 ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta aaaattttat
      421 atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca caccacagaa
      481 gtaaggttcc ttcacaaaga tcccaagctt ggatctgggg cgcccatggg cgaattcctt
      541 tttttttttt tttttttttt ttttttgctt aaacatgttt ttattatgat taaaggaaca
      601 aaagagcaca ttcacaagac ccattacata tgggtacaag gaaaacaatt tgaatagtaa
      661 tataccatat ttgccaacat accatgattg agtcaaagtt tagggagaaa tgtgaattat
      721 aagattttta taatgcatct ttaggaagtc aggaagagcc ttgtagtatc aggaacacag
      781 agaacaagca attgccttgt cagcatagga atggttggtg acagttgata atttaatctg
      841 agagattttg agtg
//
