LOCUS       sequence-126356-        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mTFP1 using His3MX6 as a selection marker in
            S. cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P,
            Pon LA
  TITLE     Characterization of Fluorescent Proteins for Three- and Four-Color 
            Live-Cell Imaging in S. cerevisiae.
  JOURNAL   PLoS One. 2016 Jan 4;11(1):e0146120. doi: 
            10.1371/journal.pone.0146120. eCollection 2016.
  PUBMED    26727004
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-4"; volume: "11(1):e0146120. doi"; pages: " 
            10.1371/journal.pone.0146120. eCollection 2016"
COMMENT     Sequence Label: pFA6-mTFP1-His3MX6
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..757
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     terminator      778..965
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(833..857)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 agcttcgtac gctgcaggtc gacggatccc cgggttaatt aacagtatgg tgagcaaggg
       61 cgaggagacc acaatgggcg taatcaagcc cgacatgaag atcaagctga agatggaggg
      121 caacgtgaat ggccacgcct tcgtgatcga gggcgagggc gagggcaagc cctacgacgg
      181 caccaacacc atcaacctgg aggtgaagga gggagccccc ctgcccttct cctacgacat
      241 tctgaccacc gcgttcgcct acggcaacag ggccttcacc aagtaccccg acgacatccc
      301 caactacttc aagcagtcct tccccgaggg ctactcttgg gagcgcacca tgaccttcga
      361 ggacaagggc atcgtgaagg tgaagtccga catctccatg gaggaggact ccttcatcta
      421 cgagatacac ctcaagggcg agaacttccc ccccaacggc cccgtgatgc agaagaagac
      481 caccggctgg gacgcctcca ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga
      541 cgtcaagcac aagctgctgc tggagggcgg cggccaccac cgcgttgact tcaagaccat
      601 ctacagggcc aagaaggcgg tgaagctgcc cgactatcac tttgtggacc accgcatcga
      661 gatcctgaac cacgacaagg actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg
      721 caactccacc gacggcatgg acgagctgta caagtaaggc gcgccacttc taaataagcg
      781 aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac
      841 aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc
      901 ctgtaggtca ggttgctttc tcaggtatag tatgaggtcg ctcttattga ccacacctct
      961 accggcagat ccgctaggga taacagggta atatagatct gtttagcttg cctcgtcccc
     1021 gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt
     1081 gcgc
//
