LOCUS       sequence-126358-        1106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mTFP1 using His3MX6 as a selection marker in
            S. cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P,
            Pon LA
  TITLE     Characterization of Fluorescent Proteins for Three- and Four-Color 
            Live-Cell Imaging in S. cerevisiae.
  JOURNAL   PLoS One. 2016 Jan 4;11(1):e0146120. doi: 
            10.1371/journal.pone.0146120. eCollection 2016.
  PUBMED    26727004
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-4"; volume: "11(1):e0146120. doi"; pages: " 
            10.1371/journal.pone.0146120. eCollection 2016"
COMMENT     Sequence Label: pFA6-mTFP1-His3MX6
FEATURES             Location/Qualifiers
     source          1..1106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(91..288)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(292..990)
                     /codon_start=1
                     /product="S. kluyveri imidazoleglycerol phosphate 
                     dehydratase"
                     /label=k-HIS3
                     /translation="MAEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDS
                     ILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHT
                     TEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSSRPFAVIDLGLKREM
                     IGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTN
                     DVPSTKGVLM"
ORIGIN
        1 cgcggccgca taggccacta gtggatctga tatcatcgat gaattcgagc tcgtttaaac
       61 tggatggcgg cgttagtatc gaatcgacag cagtatagcg accagcattc acatacgatt
      121 gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg
      181 aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa
      241 ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac ttcacatcaa
      301 aacacctttg gttgagggaa cgtcattggt gccattgcta gaaatagctt ctcttatggc
      361 aacagccaaa gccttgaacg cactctcact tctgtggtga tcgttgaaac ctctcagaca
      421 atcaacatgc aaagtaattc tggccgcctc cgcgaaactt tccaaaaagt gtggaatcat
      481 ttcagtggat aaatcaccaa tcatctctct cttcaatcca aggtcgatta cagcaaatgg
      541 tctactagat aaatcgacta cggcacgtga tagcgcctca tccaatggtg cgaacccagt
      601 accgaatctt tttacaccac ggacagcacc cattgcttct ttgaacgctt gccctaatgc
      661 gataccgcaa tcttcggtag tatggtgatc gtcaatgtgc aggtcaccaa tacattcaac
      721 aataagagac caaccagagt gttttgccaa cgcatggatc atatgatcca aaaagccaac
      781 acctgtgtga atatcgatga cctgtgactg agtagcttgg gaagctacat cgtcatcctt
      841 ctttgcagga agaatcgaat cttttatttg aatataacca ccattcagcg aaatagcgat
      901 ttgaatttta gtttcattag tgatacggga gataaacgcc ttgcgctcct gaacagtttg
      961 tttttgcttt ttttgggctg gttctgccat ggttgtttat gttcggatgt gatgtgagaa
     1021 ctgtatccta gcaagatttt aaaaggaagt atatgaaaga agaacctcag tggcaaatcc
     1081 taacctttta tatttctcta cagggg
//
