LOCUS       sequence-126360-        1147 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with mTFP1 using KanMX6 as a selection marker in 
            S. cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P,
            Pon LA
  TITLE     Characterization of Fluorescent Proteins for Three- and Four-Color 
            Live-Cell Imaging in S. cerevisiae.
  JOURNAL   PLoS One. 2016 Jan 4;11(1):e0146120. doi: 
            10.1371/journal.pone.0146120. eCollection 2016.
  PUBMED    26727004
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-4"; volume: "11(1):e0146120. doi"; pages: " 
            10.1371/journal.pone.0146120. eCollection 2016"
COMMENT     Sequence Label: pFA6-mTFP1-KanMX6
FEATURES             Location/Qualifiers
     source          1..1147
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..755
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     terminator      776..963
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(831..855)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 cttcgtacgc tgcaggtcga cggatccccg ggttaattaa cagtatggtg agcaagggcg
       61 aggagaccac aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca
      121 acgtgaatgg ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca
      181 ccaacaccat caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc
      241 tgaccaccgc gttcgcctac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca
      301 actacttcaa gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg
      361 acaagggcat cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg
      421 agatacacct caagggcgag aacttccccc ccaacggccc cgtgatgcag aagaagacca
      481 ccggctggga cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg
      541 tcaagcacaa gctgctgctg gagggcggcg gccaccaccg cgttgacttc aagaccatct
      601 acagggccaa gaaggcggtg aagctgcccg actatcactt tgtggaccac cgcatcgaga
      661 tcctgaacca cgacaaggac tacaacaagg tgaccgttta cgagagcgcc gtggcccgca
      721 actccaccga cggcatggac gagctgtaca agtaaggcgc gccacttcta aataagcgaa
      781 tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa
      841 attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct
      901 gtaggtcagg ttgctttctc aggtatagta tgaggtcgct cttattgacc acacctctac
      961 cggcagatcc gctagggata acagggtaat atagatctgt ttagcttgcc tcgtccccgc
     1021 cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc
     1081 gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat
     1141 aatcatt
//
