LOCUS       sequence-126374-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Omega plant expression vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1_omega2
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    28..52
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    131..152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(167..172,173..190,191..197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    205..221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     210..232
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     229..245
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     229..245
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    258..314
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(315..332)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(315..331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(324..346)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(540..559)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    681..703
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 atctcaacaa cacatacagg cgacttagtt tacccgccaa tatatcctgt cagatatcgg
       61 tctcaagcgt gagacgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      121 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactatatgc
      181 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      241 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta
      301 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      361 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      421 gcctgcgccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
      481 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      541 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
      601 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gcgtctcact
      661 cctgactgag accgatatcg ttacaccaca atatatcctg ccaagatcta attccgggga
      721 tcggaaatcc agaagcccga gaggttgccg cctttcgggc tttttctttt tcaaaaaa
//
