LOCUS       sequence-126382-        8850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This vector was used to generate a library of ca. 17-kb sequences of
            clones as part of a genomic sequence strategy and were then used to 
            complement mutations. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Kaneko T, Nakamura Y, Wolk CP, Kuritz T, Sasamoto S, Watanabe A, 
            Iriguchi M, Ishikawa A, Kawashima K, Kimura T, Kishida Y, Kohara M, 
            Matsumoto M, Matsuno A, Muraki A, Nakazaki N, Shimpo S, Sugimoto M, 
            Takazawa M, Yamada M, Yasuda M, Tabata S
  TITLE     Complete genomic sequence of the filamentous nitrogen-fixing 
            cyanobacterium Anabaena sp. strain PCC 7120.
  JOURNAL   DNA Res. 2001 Oct 31;8(5):205-13; 227-53.
  PUBMED    11759840
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Res."; 
            date: "2001-10-31"; volume: "8(5)"; pages: "205-13; 227-53"
COMMENT     Sequence Label: pRL838
FEATURES             Location/Qualifiers
     source          1..8850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      817..1036
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             1127..1882
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    1885..2135
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             2461..3636
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             3636..4607
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    4680..5153
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(5527..5898)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            5931..6040
                     /note="incP origin of transfer"
     misc_feature    6192..6591
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(6602..6635)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     6651..6670
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6651..6669
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6832..6849)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             6958..7617
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7024..7043)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      8795..8838
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 aagctcatgg agcggcgtaa ccgtcgcaca ggaaggacag agaaagcgcg gatctgggaa
       61 gtgacggaca gaacggtcag gacctggatt ggggaggcgg ttgccgccgc tgctgctgac
      121 ggtgtgacgt tctctgttcc ggtcacacca catacgttcc gccattccta tgcgatgcac
      181 atgctgtatg ccggtatacc gctgaaagtt ctgcaaagcc tgatgggaca taagtccatc
      241 agttcaacgg aagtctacac gaaggttttt gcgctggatg tggctgcccg gcaccgggtg
      301 cagtttgcga tgccggagtc tgatgcggtt gcgatgctga aacaattatc ctgagaataa
      361 atgccttggc ctttatatgg aaatgtggaa ctgagtggat atgctgtttt tgtctgttaa
      421 acagagaagc tggctgttat ccactgagaa gcgaacgaaa cagtcgggaa aatctcccat
      481 tatcgtagag atccgcatta ttaatctcag gagcctgtgt agcgtttata ggaagtagtg
      541 ttctgtcatg atgcctgcaa gcggtaacga aaacgatttg aatatgcctt caggaacaat
      601 agaaatcttc gtgcggtgtt acgttgaagt ggagcggatt atgtcagcaa tggacagaac
      661 aacctaatga acacagaacc atgatgtggt ctgtcctttt acagccagta ggctcgccgc
      721 agtcgagcga cagggcgaag ccctcgagtg agcgaggaag caccagggaa cagcacttat
      781 atattctgct tacacacgat gcctgaaaaa acttcccttg gggttatcca cttatccacg
      841 gggatatttt tataattatt ttttttatag tttttagatc ttctttttta gagcgccttg
      901 taggccttta tccatgctgg ttctagagaa ggtgttgtga caaattgccc tttcagtgtg
      961 acaaatcacc ctcaaatgac agtcctgtct gtgacaaatt gcccttaacc ctgtgacaaa
     1021 ttgccctcag aagaagctgt tttttcacaa agttatccct gcttattgac tcttttttat
     1081 ttagtgtgac aatctaaaaa cttgtcacac ttcacatgga tctgtcatgg cggaaacagc
     1141 ggttatcaat cacaagaaac gtaaaaatag cccgcgaatc gtccagtcaa acgacctcac
     1201 tgaggcggca tatagtctct cccgggatca aaaacgtatg ctgtatctgt tcgttgacca
     1261 gatcagaaaa tctgatggca ccctacagga acatgacggt atctgcgaga tccatgttgc
     1321 taaatatgct gaaatattcg gattgacctc tgcggaagcc agtaaggata tacggcaggc
     1381 attgaagagt ttcgcgggga aggaagtggt tttttatcgc cctgaagagg atgccggcga
     1441 tgaaaaaggc tatgaatctt ttccttggtt tatcaaacgt gcgcacagtc catccagagg
     1501 gctttacagt gtacatatca acccatatct cattcccttc tttatcgggt tacagaaccg
     1561 gtttacgcag tttcggctta gtgaaacaaa agaaatcacc aatccgtatg ccatgcgttt
     1621 atacgaatcc ctgtgtcagt atcgtaagcc ggatggctca ggcatcgtct ctctgaaaat
     1681 cgactggatc atagagcgtt accagctgcc tcaaagttac cagcgtatgc ctgacttccg
     1741 ccgccgcttc ctgcaggtct gtgttaatga gatcaacagc agaactccaa tgcgcctctc
     1801 atacattgag aaaaagaaag gccgccagac gactcatatc gtattttcct tccgcgatat
     1861 cacttccatg acgacaggat agtctgaggg ttatctgtca cagatttgag ggtggttcgt
     1921 cacatttgtt ctgacctact gagggtaatt tgtcacagtt ttgctgtttc cttcagcctg
     1981 catggatttt ctcatacttt ttgaactgta atttttaagg aagccaaatt tgagggcagt
     2041 ttgtcacagt tgatttcctt ctctttccct tcgtcatgtg acctgatatc gggggttagt
     2101 tcgtcatcat tgatgagggt tgattatcac agtttattac tctgaattgg ctatccgcgt
     2161 gtgtacctct acctggagtt tttcccacgg tggatatttc ttcttgcgct gagcgtaaga
     2221 gctatctgac agaacagttc ttctttgctt cctcgccagt tcgctcgcta tgctcggtta
     2281 cacggctgcg gcgagcgcta gtgataataa gtgactgagg tatgtgctct tcttatctcc
     2341 ttttgtagtg ttgctcttat tttaaacaac tttgcggttt tttgatgact ttgcgatttt
     2401 gttgttgctt tgcagtaaat tgcaagattt aataaaaaaa cgcaaagcaa tgattaaagg
     2461 atgttcagaa tgaaactcat ggaaacactt aaccagtgca taaacgctgg tcatgaaatg
     2521 acgaaggcta tcgccattgc acagtttaat gatgacagcc cggaagcgag gaaaataacc
     2581 cggcgctgga gaataggtga agcagcggat ttagttgggg tttcttctca ggctatcaga
     2641 gatgccgaga aagcagggcg actaccgcac ccggatatgg aaattcgagg acgggttgag
     2701 caacgtgttg gttatacaat tgaacaaatt aatcatatgc gtgatgtgtt tggtacgcga
     2761 ttgcgacgtg ctgaagacgt atttccaccg gtgatcgggg ttgctgccca taaaggtggc
     2821 gtttacaaaa cctcagtttc tgttcatctt gctcaggatc tggctctgaa ggggctacgt
     2881 gttttgctcg tggaaggtaa cgacccccag ggaacagcct caatgtatca cggatgggta
     2941 ccagatcttc atattcatgc agaagacact ctcctgcctt tctatcttgg ggaaaaggac
     3001 gatgtcactt atgcaataaa gcccacttgc tggccggggc ttgacattat tccttcctgt
     3061 ctggctctgc accgtattga aactgagtta atgggcaaat ttgatgaagg taaactgccc
     3121 accgatccac acctgatgct ccgactggcc attgaaactg ttgctcatga ctatgatgtc
     3181 atagttattg acagcgcgcc taacctgggt atcggcacga ttaatgtcgt atgtgctgct
     3241 gatgtgctga ttgttcccac gcctgctgag ttgtttgact acacctccgc actgcagttt
     3301 ttcgatatgc ttcgtgatct gctcaagaac gttgatctta aagggttcga gcctgatgta
     3361 cgtattttgc ttaccaaata cagcaatagt aatggctctc agtccccgtg gatggaggag
     3421 caaattcggg atgcctgggg aagcatggtt ctaaaaaatg ttgtacgtga aacggatgaa
     3481 gttggtaaag gtcagatccg gatgagaact gtttttgaac aggccattga tcaacgctct
     3541 tcaactggtg cctggagaaa tgctctttct atttgggaac ctgtctgcaa tgaaattttc
     3601 gatcgtctga ttaaaccacg ctgggagatt agataatgaa gcgtgcgcct gttattccaa
     3661 aacatacgct caatactcaa ccggttgaag atacttcgtt atcgacacca gctgccccga
     3721 tggtggattc gttaattgcg cgcgtaggag taatggctcg cggtaatgcc attactttgc
     3781 ctgtatgtgg tcgggatgtg aagtttactc ttgaagtgct ccggggtgat agtgttgaga
     3841 agacctctcg ggtatggtca ggtaatgaac gtgaccagga gctgcttact gaggacgcac
     3901 tggatgatct catcccttct tttctactga ctggtcaaca gacaccggcg ttcggtcgaa
     3961 gagtatctgg tgtcatagaa attgccgatg ggagtcgccg tcgtaaagct gctgcactta
     4021 ccgaaagtga ttatcgtgtt ctggttggcg agctggatga tgagcagatg gctgcattat
     4081 ccagattggg taacgattat cgcccaacaa gtgcttatga acgtggtcag cgttatgcaa
     4141 gccgattgca gaatgaattt gctggaaata tttctgcgct ggctgatgcg gaaaatattt
     4201 cacgtaagat tattacccgc tgtatcaaca ccgccaaatt gcctaaatca gttgttgctc
     4261 ttttttctca ccccggtgaa ctatctgccc ggtcaggtga tgcacttcaa aaagccttta
     4321 cagataaaga ggaattactt aagcagcagg catctaacct tcatgagcag aaaaaagctg
     4381 gggtgatatt tgaagctgaa gaagttatca ctcttttaac ttctgtgctt aaaacgtcat
     4441 ctgcatcaag aactagttta agctcacgac atcagtttgc tcctggagcg acagtattgt
     4501 ataagggcga taaaatggtg cttaacctgg acaggtctcg tgttccaact gagtgtatag
     4561 agaaaattga ggccattctt aaggaacttg aaaagccagc accctgatgc gaccacgttt
     4621 tagtctacgt ttatctgtct ttacttaatg tcctttgtta caggccagaa agcataactg
     4681 gcctgaatat tctctctggg cccactgttc cacttgtatc gtcggtctga taatcagact
     4741 gggaccacgg tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact
     4801 cgtatcgtcg gtctgattat tagtctggga ccacggtccc actcgtatcg tcggtctgat
     4861 aatcagactg ggaccacggt cccactcgta tcgtcggtct gattattagt ctgggaccat
     4921 ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca ctcgtatcgt
     4981 cggtctgatt attagtctgg aaccacggtc ccactcgtat cgtcggtctg attattagtc
     5041 tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc acgatcccac
     5101 tcgtgttgtc ggtctgatta tcggtctggg accacggtcc cacttgtatt gtcgatcaga
     5161 ctatcagcgt gagactacga ttccatcaat gcctgtcaag ggcaagtatt gacatgtcgt
     5221 cgtaacctgt agaacggagt aacctcggtg tgcggttgta tgcctgctgt ggattgctgc
     5281 tgtgtcctgc ttatccacaa cattttgcgc acggttatgt ggacaaaata cctggttacc
     5341 caggccgtgc cggcacgtta accgggctgc atccgatgca agtgtgtcgc tgtcgagggg
     5401 atcgtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
     5461 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
     5521 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
     5581 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
     5641 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
     5701 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
     5761 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
     5821 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
     5881 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
     5941 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
     6001 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
     6061 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
     6121 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aagatcgtcc
     6181 tcgacgagga cttccattgt tcattccacg gacaaaaaca gagaaaggaa acgacagagg
     6241 ccaaaaagcc tcgctttcag cacctgtcgt ttcctttctt ttcagagggt attttaaata
     6301 aaaacattaa gttatgacga agaagaacgg aaacgcctta aaccggaaaa ttttcataaa
     6361 tagcgaaaac ccgcgaggtc gccgccccgt aacctgtcgg atcaccggaa aggacccgta
     6421 aagtgataat gattatcatc tacatatcac aacgtgcgtg gaggccatca aaccacgtca
     6481 aataatcaat tatgacgcag gtatcgtatt aattgatctg catcaactta acgtaaaaac
     6541 aacttcagac aatacaaatc agcgacactg aatacggggc aacctcatgt cctcatcgct
     6601 aataacttcg tatagcatac attatacgaa gttatattcg atgcggccgc taatacgact
     6661 cactataggg agaagcttgc atgcctgcag gtcgactcta ggggtaccga gctcgaattc
     6721 atcgatctcg taacccgggc ccgcggatgc atatgatcag atcttaaggc ctaggtctag
     6781 acgtcgtacg tgcactcgag cccgggcgcg ccggaccggc cggcagggat cctatagtgt
     6841 cacctaaatc gtatgcggcc gctcgtaaga ggttccaact ttcaccataa tgaaataaga
     6901 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg
     6961 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
     7021 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
     7081 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
     7141 attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag
     7201 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
     7261 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
     7321 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
     7381 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
     7441 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
     7501 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc
     7561 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt
     7621 ttttaaggca gttattggtg cccttaaacg cctggtgcta cgcctgaata agtgataata
     7681 agcggatgaa tggcagaaat tcgatgaatt cgcgtgctat aattatacta attttataag
     7741 gaggaaaaaa tatgggcatt tttagtattt ttgtaatcag cacagttcat tatcaaccaa
     7801 acaaaaaata agtggttata atgaatcgtt aataagcaaa attcatataa ccaaattaaa
     7861 gagggttata atgaacgaga aaaatataaa acacagtcaa aactttatta cttcaaaaca
     7921 taatatagat aaaataatga caaatataag attaaatgaa catgataata tctttgaaat
     7981 cggctcagga aaaggccatt ttacccttga attagtaaag aggtgtaatt tcgtaactgc
     8041 cattgaaata gaccataaat tatgcaaaac tacagaaaat aaacttgttg atcacgataa
     8101 tttccaagtt ttaaacaagg atatattgca gtttaaattt cctaaaaacc aatcctataa
     8161 aatatatggt aatatacctt ataacataag tacggatata atacgcaaaa ttgtttttga
     8221 tagtatagct aatgagattt atttaatcgt ggaatacggg tttgctaaaa gattattaaa
     8281 tacaaaacgc tcattggcat tacttttaat ggcagaagtt gatatttcta tattaagtat
     8341 ggttccaaga gaatattttc atcctaaacc taaagtgaat agctcactta tcagattaag
     8401 tagaaaaaaa tcaagaatat cacacaaaga taaacaaaag tataattatt tcgttatgaa
     8461 atgggttaac aaagaataca agaaaatatt tacaaaaaat caatttaaca attccttaaa
     8521 acatgcagga attgacgatt taaacaatat tagctttgaa caattcttat ctcttttcaa
     8581 tagctataaa ttatttaata agtaagttaa gggatgcagg tcgagtaccc gtacaagcct
     8641 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     8701 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     8761 atggtcagcg aaccaatcag cagggtcatc gctagaaatc atccttagcg aaagctaagg
     8821 atttttttta tctgaattaa ttctcgcgat
//
