LOCUS       sequence-126384-        3525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid level 1 Omega plant expression vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1_omega2R
FEATURES             Location/Qualifiers
     source          1..3525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    87..108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(123..128,129..146,147..153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    161..177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     166..188
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     185..201
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     185..201
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    214..270
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(271..288)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(271..287)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(280..302)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(496..515)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    635..659
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(750..1338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(830..849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1509..2300)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2944..3379
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    join(3506..3525,1..3)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 taacaagctt ggtctcagtc aagcgtgaga cgcagctggc acgacaggtt tcccgactgg
       61 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      121 gctttacact atatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      181 cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca ggtcgactct
      241 agaggatccc cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg
      301 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      361 gcgtaatagc gaagaggcct gcgccgatcg cccttcccaa cagttgcgca gcctgaatgg
      421 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
      481 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
      541 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
      601 agctgtgcgt ctcactccgc ttgagaccaa gctttgacag gatatattgg cgggtaaact
      661 aagtcgctgt atgtgtttgt ttgagatctc atgtgagcaa aaggccagca aaaggccagg
      721 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      781 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      841 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      901 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
      961 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1021 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1081 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1141 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1201 ggtatctgcg ctctgctgaa gccagttacc ttcggaagaa gagttggtag ctcttgatcc
     1261 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1321 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1381 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1441 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatgt gtaacattgg
     1501 tctagtgatt atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct
     1561 tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta
     1621 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
     1681 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
     1741 actacatttc gctcatcacc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
     1801 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
     1861 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
     1921 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
     1981 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
     2041 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
     2101 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
     2161 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
     2221 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
     2281 gcgatcaccg cttccctcat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta
     2341 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     2401 gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc
     2461 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     2521 cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgccttc
     2581 atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt
     2641 ctgtcctggc tggaacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
     2701 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc
     2761 tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca
     2821 acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca
     2881 aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcga
     2941 tccccatcca acagcccgcc gtcgagcggg cttttttatc cccggaagcc tgtggataga
     3001 gggtagttat ccacgtgaaa ccgctaatgc cccgcaaagc cttgattcac ggggctttcc
     3061 ggcccgctcc aaaaactatc cacgtgaaat cgctaatcag ggtacgtgaa atcgctaatc
     3121 ggagtacgtg aaatcgctaa taaggtcacg tgaaatcgct aatcaaaaag gcacgtgaga
     3181 acgctaatag ccctttcaga tcaacagctt gcaaacaccc ctcgctccgg caagtagtta
     3241 cagcaagtag tatgttcaat tagcttttca attatgaata tatatatcaa ttattggtcg
     3301 cccttggctt gtggacaatg cgctacgcgc accggctccg cccgtggaca accgcaagcg
     3361 gttgcccacc gtcgagcgcc tttgcccaca acccggcggc cggccgcaac agatcgtttt
     3421 ataaattttt ttttttgaaa aagaaaaagc ccgaaaggcg gcaacctctc gggcttctgg
     3481 atttccgatc cccggaatta gatcttggca ggatatattg tggtg
//
