LOCUS       sequence-126385-        1305 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal portion of GFP along with VMA intein for 
            protein splicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: pcDNA3-NGFP-VMAn-FKBP
FEATURES             Location/Qualifiers
     source          1..1305
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             985..1305
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcatcctgaa gggcgaattc cttaagggct gctttgccaa gggtaccaat
      421 gttttaatgg cggatgggtc tattgaatgt attgaaaaca ttgaggttgg taataaggtc
      481 atgggtaaag atggcagacc tcgtgaggta attaaattgc ccagaggaag agaaactatg
      541 tacagcgtcg tgcagaaaag tcagcacaga gcccacaaaa gtgactcaag tcgtgaagtg
      601 ccagaattac tcaagtttac gtgtaatgcg acccatgagt tggttgttag aacacctcgt
      661 agtgtccgcc gtttgtctcg taccattaag ggtgtcgaat attttgaagt tattactttt
      721 gagatgggcc aaaagaaagc ccccgacggt agaattgttg agcttgtcaa ggaagtttca
      781 aagagctacc caatatctga ggggcctgag agagccaacg aattagtaga atcctataga
      841 aaggcttcaa ataaagctta ttttgagtgg actattgagg ccagagatct ttctctgttg
      901 ggttcccatg ttcgtaaagc tacctaccag acttacgctc caattcttta ttctagaaac
      961 aacggcaacg ggaacggcac tagaggagtg caggtggaaa ccatctcccc aggagacggg
     1021 cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat gcttgaagat
     1081 ggaaagaaat ttgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag
     1141 caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc
     1201 aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca
     1261 ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaa
//
