LOCUS       sequence-126386-         384 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal portion of GFP along with VMA intein for 
            protein splicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: pcDNA3-NGFP-VMAn-FKBP
FEATURES             Location/Qualifiers
     source          1..384
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcatcctgaa gggc
//
