LOCUS       sequence-126390-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Omega plant expression vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB3_omega1R
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    491..515
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    603..624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(639..644,645..662,663..669)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    677..693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     682..704
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     701..717
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     701..717
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    730..786
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(787..804)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(787..803)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(796..818)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtgcgtcacc cggcaacctt gggtagcagc gaagtcgagg catttctgtc ctggctggaa
       61 cagaacttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
      121 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
      181 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
      241 ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
      301 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
      361 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
      421 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
      481 ggctggctgg tggcaggata tattgtggtg taaacataac gaattcggtc tcaggagagc
      541 gtgagacgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      601 attaatgtga gttagctcac tcattaggca ccccaggctt tacactatat gcttccggct
      661 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      721 gattacgcca agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc
      781 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      841 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcctgcgc
      901 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
//
