LOCUS       sequence-126395-       12442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively express mitochondrial localized PhyB in Saccharomyces
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12442)
  AUTHORS   Jost AP, Weiner OD
  TITLE     Probing Yeast Polarity with Acute, Reversible, Optogenetic 
            Inhibition of Protein Function.
  JOURNAL   ACS Synth Biol. 2015 Jun 2.
  PUBMED    26035630
REFERENCE   2  (bases 1 to 12442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Jun 2."
COMMENT     Sequence Label: PhyB-mCherry-Tom7
FEATURES             Location/Qualifiers
     source          1..12442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..683
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        3293..3997
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             6787..7491
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6929..6947)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7197..7216
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8989..9008)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8990..9008)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9015..9032)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9015..9031)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9024..9046)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9172..9627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9259..9278)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9469..9490
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9983..10002)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10102..10124
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10162..10180)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10265..10369
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10370..10438,10439..11230)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10588..10607)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11401..11989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11890..11909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12143..12160
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12277..12298
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12313..12318,12319..12336,12337..12343)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12351..12367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12356..12378
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12375..12391
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12375..12391
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        12412..12430
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta
       61 catacctaat attattgcct tattaaaaat ggaatcccaa cgattacatc aaaatccaca
      121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
      181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
      241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
      301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
      361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
      421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
      481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
      541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
      601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
      661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa
      721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa
      781 aatatgaaag tttaagtttt cagttcatcc ccattgtagt tatcaaattt ctgtgtttcc
      841 cgtatatgtg tgctctgtat tcatcgctat cttgaaactg gttatgatcg ttgcgcatgt
      901 gacctgtatc gctcaaaaga gcccggcacc ggtaccgggc aaaagcccct agcaagctaa
      961 aatgattcaa ctttcgtagt aatttgttca accggtcaca gtaaagtcaa tgactaactt
     1021 ttgaatcgag gatgtatttc caattgaaag caatgtatat ataaaccatt gtgttgaagc
     1081 ttgtcttaca gttttcttgt atcctcccat tgttcatata gtacttaaag taccataatc
     1141 cttaaagaac aaacttgaaa gagaatacac tatacacaac taataaataa agaataatca
     1201 tggctgtgac caagacaatt gtagttctac caggtgacca tgttggtcaa gaaatcactg
     1261 aagaggccat taaagtttta aatgctattc aggaatgtcg tccagacaag gtcaatttca
     1321 agtttgatca tcatttgatc ggtggtgctg caattgatgc cactggtgtt ccattaccag
     1381 acgaagcttt ggaggcctct aagaaagctg atgccgtgct tctgggtgct gttggtggtc
     1441 caaaatgggg tactggcgct gtcagaccag aacaaggtct tttgaaaatc cgtaaggagt
     1501 tgcaattgta tgctaatcta agaccatgta attttgcatc tgattcctta ctagatctat
     1561 cgccattgaa gcctgaaatt gcaagaggta cagatttcgt cgttgttaga gaactagtgg
     1621 gtggtattta tttcggggag agaaaagaag atgaaggtga tggtgtcgcc tgggatagcg
     1681 aaaagtattc tgtgcccgaa gttcaaagaa tcactagaat ggctgctttc atggctctgc
     1741 aacacaaccc acctttacct atttggtctc tagataaagc caacgttcta gcatcatcaa
     1801 gattatggag aaaaacagtg gaagagacga ttaagaatga attcccacaa ttgactgttc
     1861 aacatcaatt gattgattcc gctgctatga tcttggttaa gaacccaact catctaaatg
     1921 gtattattat cacaaacaat atgtttggtg atattatctc cgatgaagcc tccgtgatcc
     1981 caggttcgtt aggattactt ccatctgcct ccttggcatc tcttccagat aagaatacgg
     2041 catttggttt atatgagcca tgtcatggct ctgctccaga cttaccaaag ggaaaggtta
     2101 accccgtagc aacaatccta tctgctgcta tgatgttgaa gttgtctctg gacctgttcg
     2161 aagaaggtga aattatagaa caagcagtca agaaagtgtt ggattctggt atcagaacag
     2221 cggatctaaa gggtaccaac tctactactg aggtcggtga tgcagtagcg aaagctgtca
     2281 gggaactatt agcttagact tagtagatgt acagttgcat acataagtct gaacagaaag
     2341 aagtaaatat catatctagt tcggtttaga aatcagtgac tatattggtt ttgaccagat
     2401 cgcaatcctt gcaagcattt agtaatgact atcaaaccgg tgaatcatct gataaggcta
     2461 tattagcttg tgcattcgca tgtatcggga aacgaacggt accgggccct aaaacaagaa
     2521 gagggttgac tacatcacga tgagggggat cgaagaaatg atggtaaatg aaataggaaa
     2581 tcaaggagca tgaaggcaaa agacaaatat aagggtcgaa cgaaaaataa agtgaaaagt
     2641 gttgatatga tgtatttggc tttgcggcgc cgaaaaaacg agtttacgca attgcacaat
     2701 catgctgact ctgtggcgga cccgcgctct tgccggcccg gcgataacgc tgggcgtgag
     2761 gctgtgcccg gcggagtttt ttgcgcctgc attttccaag gtttaccctg cgctaagggg
     2821 cgagattgga gaagcaataa gaatgccggt tggggttgcg atgatgacga ccacgacaac
     2881 tggtgtcatt atttaagttg ccgaaagaac ctgagtgcat ttgcaacatg agtatactag
     2941 aagaatgagc caagacttgc gagacgcgag tttgccggtg gtgcgaacaa tagagcgacc
     3001 atgaccttga aggtgagacg cgcataaccg ctagagtact ttgaagagga aacagcaata
     3061 gggttgctac cagtataaat agacaggtac atacaacact ggaaatggtt gtctgtttga
     3121 gtacgctttc aattcatttg ggtgtgcact ttattatgtt acaatatgga agggaacttt
     3181 acacttctcc tatgcacata tattaattaa agtccaatgc tagtagagaa ggggggtaac
     3241 acccctccgc gctcttttcc gatttttttc taaaccgtgg aatatttcgg atatcctttt
     3301 gttgtttccg ggtgtacaat atggacttcc tcttttctgg caaccaaacc catacatcgg
     3361 gattcctata ataccttcgt tggtctccct aacatgtagg tggcggaggg gagatataca
     3421 atagaacaga taccagacaa gacataatgg gctaaacaag actacaccaa ttacactgcc
     3481 tcattgatgg tggtacataa cgaactaata ctgtagccct agacttgata gccatcatca
     3541 tatcgaagtt tcactaccct ttttccattt gccatctatt gaagtaataa taggcgcatg
     3601 caacttcttt tctttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca
     3661 atgacaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga cagcaccaac
     3721 agatgtcgtt gttccagagc tgatgagggg tatctcgaag cacacgaaac tttttccttc
     3781 cttcattcac gcacactact ctctaatgag caacggtata cggccttcct tccagttact
     3841 tgaatttgaa ataaaaaaaa gtttgctgtc ttgctatcaa gtataaatag acctgcaatt
     3901 attaatcttt tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg
     3961 cacaatattt caagctatac caagcataca atcaactatc tcatatacaa agcaatggtt
     4021 tccggagtcg ggggtagtgg cggtggccgt ggcggtggcc gtggcggaga agaagaaccg
     4081 tcgtcaagtc acactcctaa taaccgaaga ggaggagaac aagctcaatc gtcgggaacg
     4141 aaatctctca gaccaagaag caacactgaa tcaatgagca aagcaattca acagtacacc
     4201 gtcgacgcaa gactccacgc cgttttcgaa caatccggcg aatcagggaa atcattcgac
     4261 tactcacaat cactcaaaac gacgacgtac ggttcctctg tacctgagca acagatcaca
     4321 gcttatctct ctcgaatcca gcgaggtggt tacattcagc ctttcggatg tatgatcgcc
     4381 gtcgatgaat ccagtttccg gatcatcggt tacagtgaaa acgccagaga aatgttaggg
     4441 attatgcctc aatctgttcc tactcttgag aaacctgaga ttctagctat gggaactgat
     4501 gtgagatctt tgttcacttc ttcgagctcg attctactcg agcgtgcttt cgttgctcga
     4561 gagattacct tgttaaatcc ggtttggatc cattccaaga atactggtaa accgttttac
     4621 gccattcttc ataggattga tgttggtgtt gttattgatt tagagccagc tagaactgaa
     4681 gatcctgcgc tttctattgc tggtgctgtt caatcgcaga aactcgcggt tcgtgcgatt
     4741 tctcagttac aggctcttcc tggtggagat attaagcttt tgtgtgacac tgtcgtggaa
     4801 agtgtgaggg acttgactgg ttatgatcgt gttatggttt ataagtttca tgaagatgag
     4861 catggagaag ttgtagctga gagtaaacga gatgatttag agccttatat tggactgcat
     4921 tatcctgcta ctgatattcc tcaagcgtca aggttcttgt ttaagcagaa ccgtgtccga
     4981 atgatagtag attgcaatgc cacacctgtt cttgtggtcc aggacgatag gctaactcag
     5041 tctatgtgct tggttggttc tactcttagg gctcctcatg gttgtcactc tcagtatatg
     5101 gctaacatgg gatctattgc gtctttagca atggcggtta taatcaatgg aaatgaagat
     5161 gatgggagca atgtagctag tggaagaagc tcgatgaggc tttggggttt ggttgtttgc
     5221 catcacactt cttctcgctg cataccgttt ccgctaaggt atgcttgtga gtttttgatg
     5281 caggctttcg gtttacagtt aaacatggaa ttgcagttag ctttgcaaat gtcagagaaa
     5341 cgcgttttga gaacgcagac actgttatgt gatatgcttc tgcgtgactc gcctgctgga
     5401 attgttacac agagtcccag tatcatggac ttagtgaaat gtgacggtgc agcatttctt
     5461 taccacggga agtattaccc gttgggtgtt gctcctagtg aagttcagat aaaagatgtt
     5521 gtggagtggt tgcttgcgaa tcatgcggat tcaaccggat taagcactga tagtttaggc
     5581 gatgcggggt atcccggtgc agctgcgtta ggggatgctg tgtgcggtat ggcagttgca
     5641 tatatcacaa aaagagactt tcttttttgg tttcgatctc acactgcgaa agaaatcaaa
     5701 tggggaggcg ctaagcatca tccggaggat aaagatgatg ggcaacgaat gcatcctcgt
     5761 tcgtcctttc aggcttttct tgaagttgtt aagagccgga gtcagccatg ggaaactgcg
     5821 gaaatggatg cgattcactc gctccagctt attctgagag actcttttaa agaatctgag
     5881 gcggctatga actctaaagt tgtggatggt gtggttcagc catgtaggga tatggcgggg
     5941 gaacagggga ttgatgagtt aggtgcagtt gcaagagaga tggttaggct cattgagact
     6001 gcaactgttc ctatattcgc tgtggatgcc ggaggctgca tcaatggatg gaacgctaag
     6061 attgcagagt tgacaggtct ctcagttgaa gaagctatgg ggaagtctct ggtttctgat
     6121 ttaatataca aagagaatga agcaactgtc aataagcttc tttctcgtgc tttgagaggg
     6181 gacgaggaaa agaatgtgga ggttaagctg aaaactttca gccccgaact acaagggaaa
     6241 gcagtttttg tggttgtgaa tgcttgttcc agcaaggact acttgaacaa cattgtcggc
     6301 gtttgttttg ttggacaaga cgttactagt cagaaaatcg taatggataa gttcatcaac
     6361 atacaaggag attacaaggc tattgtacat agcccaaacc ctctaatccc gccaattttt
     6421 gctgctgacg agaacacgtg ctgcctggaa tggaacatgg cgatggaaaa gcttacgggt
     6481 tggtctcgca gtgaagtgat tgggaaaatg attgtcgggg aagtgtttgg gagctgttgc
     6541 atgctaaagg gtcctgatgc tttaaccaag ttcatgattg tattgcataa tgcgattggt
     6601 ggccaagata cggataagtt ccctttccca ttctttgacc gcaatgggaa gtttgttcag
     6661 gctctattga ctgcaaacaa gcgggttagc ctcgagggaa aggttattgg ggctttctgt
     6721 ttcttgcaaa tcccgagcga attcgatagt gctggtagtg ctggtagtgc tggtggtagt
     6781 tccctagtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     6841 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     6901 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     6961 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     7021 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     7081 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     7141 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     7201 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     7261 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     7321 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     7381 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     7441 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gggtagcgat
     7501 agcgcaggca gtgctggtaa ggctagcagt ttcctaccat cttttatctt aagcgatgaa
     7561 tctaaagaac gtatttccaa aattttaact ttgactcata atgtagcaca ttatggctgg
     7621 atcccatttg ttttgtattt gggctgggca cacacttcta atagaccaaa ctttttgaac
     7681 ttactgtctc cgttaccaag tgtttaagcg gccgccaccg cggtggagct ctaagcaaat
     7741 agctaaatta tatacgaatt aatattatga ttaagtgttt acgtgagtgc gatattttta
     7801 ttactatctt atacagttgt atatactcta taaaatgagt tgtctattaa ttaacgcgat
     7861 gaatgctttc tgggtttacc tctccaacaa ctctagttta cttctcaata cattcaattg
     7921 tatttgattt gtcaatactt catcattaat caattctata gttttgtttt tctcgtttat
     7981 ttccaaattt aatgcatcaa ttttattatt caatttgtcg ttgattttgg ttaatgattt
     8041 tatggtttga tctctggcat tgattgtttg tgttagtttt tcattattga taattaaatt
     8101 atttaagtta gttatcaact cggtgttttc aagtttcaag ttttcaattt ctttagagtt
     8161 tattagattt gtcaaagttt ctgaattgct tgattggtcc tgtagaagag tatttgttgt
     8221 tgtggataat tgattcaatt tttgagacaa ttgctggaag gcgttgaaat atctagcatc
     8281 aatctcatgg tttttttccc gagagtctcg tagattcaat tgttttaata tatcttggga
     8341 ccactcttga tttgaactca tggaaattaa actgggtgtt gtgttgtggt gtaatgattg
     8401 tacccccttt gcttataatt gtgtggccac cgaagtcggt gatgctgtcg ccgaagaagt
     8461 taagaaaatc cttgcttaaa aagattctct ttttttatga tatttgtaca taaactttat
     8521 aaatgaaatt cataatagaa acgacacgaa attacaaaat ggaatatgtt catagggtag
     8581 acgaaactat atacgcaatc tacatacatt tatcaagaag gagaaaaagg aggatgtaaa
     8641 ggaatacagg taagcaaatt gatactaatg gctcaacgtg ataaggaaaa agaattgcac
     8701 tttaacatta atattgacaa ggaggagggc accacacaaa aagttaggtg taacagaaaa
     8761 tcatgaaact atgattccta atttatatat tggaggattt tctctaaaaa aaaaaaaata
     8821 caacaaataa aaaacactca atgacctgac catttgatgg agtttaagtc aatacctcct
     8881 tgaaccattt cccataatgg tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc
     8941 ttaacgacgt aggccggcca tttaaatgtt taaacggcca cgctggcccc ctatagtgag
     9001 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     9061 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     9121 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct
     9181 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     9241 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     9301 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     9361 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     9421 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     9481 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     9541 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     9601 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     9661 cggtatttca caccgcatag ggtaataact gatataatta aattgaagct ctaatttgtg
     9721 agtttagtat taggatctct acccttggcg aaaagtcctc tgccaacaat gatgatatct
     9781 gatccaccac ttacaacttc gtcgacggtt ctgtactgct gacccaatat gcgtatatat
     9841 accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca
     9901 aaaaaatttc aaggaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa
     9961 aggtggtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
    10021 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
    10081 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
    10141 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
    10201 taataatggt ttcttagacg tcggcgcgcc gaggacgtca ggtggcactt ttcggggaaa
    10261 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    10321 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
    10381 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
    10441 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
    10501 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
    10561 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
    10621 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
    10681 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
    10741 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    10801 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    10861 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
    10921 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    10981 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    11041 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    11101 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    11161 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    11221 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    11281 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    11341 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    11401 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    11461 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    11521 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    11581 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    11641 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    11701 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    11761 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    11821 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    11881 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    11941 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    12001 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    12061 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    12121 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
    12181 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
    12241 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    12301 ggcaccccag gctttacact ttatgcttcc ggctcctatg ttgtgtggaa ttgtgagcgg
    12361 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg gaattaaccc
    12421 tcactaaagg gaacaaaagc tg
//
