LOCUS       sequence-126400-        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentivirus expressing iRFP670, Cre, and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: pTRE:iRFP670-EFS:Cre-2A-GFP
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(104..124)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(175..386)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 cctggcgatc cctgaacatg tccatcaggt tcttgcgaac ctcatcactc gttgcatcga
       61 ccggtaatgc aggcaaattt tggtgtacgg tcagtaaatt ggacaccttc ctcttcttct
      121 tgggcatggt ccgcttaagc tgtagcgcta atgtcgggtc ccgcgtcacg acacctgtgt
      181 tctggcggca aacccgttgc gaaaaagaac gttcacggcg actactgcac ttatatacgg
      241 ttctccccca ccctcgggaa aaaggcggag ccagtacacg acatcacttt cccagtttac
      301 cccgcgccac cttctctagg caccggttca attgccgacc cctcccccca acttctcggg
      361 gactgtgggc gatgtgcgct ctgcccactg acgggcaccg gagccactcg aaggcctcgg
      421 gattcctagg aacagcggtt ttcacctctg gtgatgagcg gcagtgaagt gaagagacag
      481 gaagtcggca aacatttcag ctgccacgcg ttgggccatg gggacagcgc gaggttcata
      541 atgatgacag ataatcagtc cccagagagt tccgtcgatg atgatggaca ggctcatgga
      601 agctgacaca cccatgtttc tcaggaattc caggtggcat ggagagatgc ttcggagatg
      661 agcaaaggaa aggtcgaggg cggcaccact agcgtcgtgc tcgggcacga tgcggctgct
      721 aatgcccctg gagtcgctca ccacccgtat agcatttttg agatagagca acctagcttg
      781 ctggggtacc aggctggcag gaaagtgttg gccaagaaat gactccaggt cggagcgctt
      841 ggcctctcct atcaccatac cggacccgtc gtctgcaaat cggtaaagca taacgcggtg
      901 gtatcccagc attgcttgca aatacctagg gactcttgct gccatctcct ccagtgactt
      961 caattctttc gtcctggcga tgatctgcct ggtcaatctc aatgggttat cggcctgttc
     1021 agctgcggcc ggttcgaact caatg
//
